Research Of Association Between β Fibrinogen Gene-455G/A Polymorphism And Acute Myocardial Infarction

Objectives: To analyze the frequency of -fibrinogen gene -455 genotypes in acute myocardial infarction (AMI) group and control group. To investigate wether the -455G/A polymorphisms were associatied with the risk of AMI. To demonstrate wether possession of the -455A allele is a genetic risk of AMI. Subject and Methods: Forty patients suffered from acute myocardial infarction within one week, as defined by World Health Organization criteria. And forty control subjected were recruited. The participators in cnotrol group had no histories of coronary heart disease, ischemic stroke and peripheral arterial disease. The -455 polymorphism in the -fibrinogen gene were detected by polymerase chair reaction amplication of fragments of DNA by using specific oligonucleotide primers in a thermal cycler. Polymerase chain reaction products were subjected to 3 hours digestion by 4U HaeIII restriction enzymes at 37 C. Restricted DNA products were then separated by useng 3% agarose gel electrophoresis and visuslized by UV light. Interactions between these changes and other factors were analyzed with SPSS statistical software 11.5. Result: (1) There were signiferences diferrences in fibrinogen -455A allele distributions between AMI and in control groups, the frequence of -455A allele were higher in AMI group than control group. (2) There were signiferences diferrences in fibrinogen -455 genotype distributions between AMI and control groups, the frequence of -455AA and GG genotypes in AMI group were higher than that in control group. (3) There were no signiferences diferrences in hypertension and diabates groups in three fibrinogen -455 genotypes. (4) There were no signiferences diferrences in free plasma blood sugar, total cholesterol and triglyceride levels in three fibrinogen -455 genotypes. (5) Levels of sugar and blood triglyceride were higher in patients than that in control subjects. (5) fibrinogen-455GA and AA genotypes were contribute with acute myocardial infarction. (6)Sex and blood sugar were contribute with acute myocardial infarction. Conclusion: (1) Fibrinogen β -455G/A polymorphisms is a genetic risk factor of acute myocardial infarction. (2) Fibrinogen β -455G/A polymorphisms have no associations with hypertension and diabetes. (3) Fibrinogen 3 -455G/A polymorphisms have no associations with the metabolism of sugar, cholesterol and triglyceride. (4) Sex, blood sugar and triglyceride have associations with AMI.