The Relationship Between Fibrinogen, Polymorphism β-455G/A, Hs-CRP And Acute Myocardial Infarction

Objective: To investigate the relationship between gene frequency,allele frequency of Fgβ455G/A and pathological change degree of myocardial infarction(MI),blood plasma Fg,CRP; polymorphism of Fgβ455G/A and environmental factors including gender,age,body weight,smoking,alcohol,blood pressure,blood fat,myocardium enzyme,Blood viscosity,white blood count,blood platelets count and so on ,also to discuss the mechanism about abnomal level of Fg and CRP.Methods:Select the specimen of 72 patients suffering from MI and 78 for coronary artery disease in(control group) China-Japan Union hospital of JiLin University.Adopt molecular biology–restricted length polymorphism reaction including extracting genome DNA from leucocytes in the venous blood(2% EDTA coagulation),amplification of objective gene, cutting amplification products by restriction enzyme HaeⅢ(37℃,water bath for 1 hour)and observe results by agarose gel electrophoresis under extreme ultraviolet lamp.Test the level of Fg and CRP by coagulogram analysator ACL900 and beckman autoanalyser. Analyze the two groups (MI and Control) by statistic software SPSS 11.5, Compare two groups on gene frequency,allele frequency of Fgβ455G/A ,pathological change degree of MI byⅩ2 test,and Fg,CRP and other items including gender,age,body weight,smoking,alcohol,blood pressure,blood fat,myocardium enzyme,Blood viscosity,white blood count,blood platelets count by T test.Results:1. There is significant difference on polymorphism of DNAFgβ455 G/G,G/A,A/A,allele frequency of G and A between two groups(P<0.05), polymorphism of DNAFgβ455 in MI is G/G51.4%,G/A40.3%,A/A8.3% and in CHD is G/G74.3%,G/A24.4%,A/A1.3%;2.There is no significant difference between polymorphism of DNAFgβ455 of MI and pathological change degree of coronary arteriongraphy (P>0.05);3. The level of Fg of gene type G/A and A/A is much higher than G/G in both group MI and CHD(P<0.005),and The level of Fg in group MI is much higher than CHD.4.There is no significant difference between CRP and polymorphism of DNAFgβ455 in both group MI and CHD(P>0.05),and the concentration of CRPin group MI is much higher than CHD(P<0.005);5.There is no significant difference between polymorphism of DNAFgβ455 of MI and blood fat,AST,LDH,CK,blood viscosity,WBC,PLT(P>0.05);6. There is significant difference on G/G and G/A between age≤50 and age>50 in MI(P<0.05);7. There is no significant difference between polymorphism of DNAFgβ455 and body weight,smoking,blood pressure(P>0.05);8. There is significant difference on CHOL,TG,LDL,AST,LDH,CK,WBC,PLT, whole blood apparent viscosity between two groups,and there are also positive correlation between Fg,CRP and environmental factors.Conclusions:1. Allelotype G/A,A/A of Fgβ455 is probably heritage risk factor of AMI;2The level of Fg is affected by polymorphism of Fgβ455;3 the high sensitive CRP is not affected by polymorphism of Fgβ455;4.There is There is significant difference between Fg,hs-CRP and environmental factors such as age,smoking and so on;5.The union of Fg and hs-CRP can be useful for anticipation and diagnose AMI as dangerous factors.