| Purposeactue necrotizing pancreatitis ( ANP) is a fatal disease whose mortality is more than 20%. One of the pathogenic mechanisms of pancreatic necrosis may be local vasospasm and intravascular coagulation as a consequence of microcir-culatory disorder, which also occur in other organs in case severe information reaction occurs. Acute intestinal mucosal lesion plays a major role in those patients'deaths with ANP. This study is on the basis of rats'ANP models to detect the treatment effect of growth hormone (GH) on intestinal mucosal lesionby observing the dynamic changes of intestinal mucosal blood flow and interleukin - 1 B(IL-1β).Materials and methods1. Experimental animals and models preparation: 192 Wistar male rats were divided randomly into three groups: normal control group ( C ) , severe acute pancreatitis group (P) and treatment group (T). Each of them was divided av-eragely into four groups according to 2 hours, 6 hours, 12 hours and 24 hours after models preparation with each group separating into A and B as follows: A was to detect intestinal mucosal blood flow and B was to detect IL -1β activity by radioimmunoassay method and observe intestinal mucosal histologic changes. ANP models were established by retrograde injecting 5% sodium taurocholate into cholangiopancreatic duct, and control group was by overturning duodenum on-ly2. Statistical management: SPSS11.5 statistical software was used.Results1. Intestinal blood flow: Comparing with the intestinal blood flow in C group, that in P group decreased markedly in all phases (P <0. 05) , whereas that in T group had no obvious changes ( P > 0. 05 ). The intestinal mucosal blood flow in P group decreased ( P < 0. 05 ) in all phases in comparision with that in T group (P <0. 05).2. IL - 1β level: IL - 1β activity in P and T groups increased significantly in all phases in comparasion with that in C group ( P < 0. 05 ). IL-1β activity was higherin all phases in P group than that in T group.3. Pathological changes: No remarkable pathological changes were observed in the intestinal mucosal of the rats in C group, intestinal mucosal interstitial congestion, a little neutrophil infiltration, microembolization, alveolar edema and alveolar cavity bleeding occurred in ANP group, and aggravated as time passed, intestinal mucosal pathological changes ameliorated obviously in T group comparing to that in ANP group ( P < 0. 05 ) , and it was minor in comparasion with that in C group ( P > 0.05).4. Correlation analysis: Pearson correlation analysis showed that intestinal mucosal blood flow was highly negatively correlated with serum IL - 1β activity in P group (r= -0.610, P=0.000) ; Spearman correlation analysis discovered that intestinal mucosal blood flow was highly negatively correlated with serum IL-1β activity and positively correlated with injury degree of intestinal mucosal in P group (r= -0.468, 0.666, P<0.01)DiscussionIn the earlier period (2h) of ANP , intestinal mucosal blood flow markedly decreases and at the same time intestinal mucosal damage occurs, and they are highly correlated negatively, intestinal mucosal blood flow increased and the injury degree alleviated after using growth hormone , so we infered that ameliora-ting microcirculation can protect intestinal mucosal. In this experiment, the serum IL -1β markedly decreased and the intestinal mucosal pathological changes alleviated after using growth hormone ,but the level of serum IL -1β was higher than normal value. So we can conclude that growth hormone cannot affect the level of cell factors directly, but it can indirectly alleviate inflammation reaction of BT.Microthrombosis plays a baton role in MODS occurrence of ANP , which is because inflammation reaction imbalance as a consequence of preliminary strike factors and immune regulation energy abnormity and is also the direct reason of organs dysfunction, even failure. As a kind of fibrin - lysis agent, growth hormone can directly activate plasminogen to turn into fibrinolys...
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