Studies On Extraction Of Saponin And Quality Standard Of Radix Polygalae(Yuanzhi)

Saponin is the main sedative efficacious position of Radix Polygalae(Yuanzhi). Using total saponin content as a marker, the optimum extraction technology of Radix Polygalae(Yuanzhi) was selected with the orthogonal experiment. The content of saponin in Radix Polygalae(Yuanzhi) was determined by UV-VIS spectrophotometry after it has been coloured. The optimum extraction process is as follows: adding 6 times 85% ethanol, reflux-extraction 3 times, 120min for each time.Macroporous adsorption resin is a kind of nonionic macromolecule polymer, which widely used in the pharmacy field especially for adsorption and purification saponin of traditional Chinese medicine. Using macroporous adsorption resin isolation and purification of saponin from Radix Polygalae(Yuanzhi) is the first time. Studied the Influencing factors of macroporous adsorption resin, such as the adsorptive capacity, elution ratio, substance quantity and purified ratio, the optimum purification process of saponin is as follows: D101 macroporous adsorption resin , eluted with 3BV water, 3BV 30% ethanol and 3BV 70% ethanol in turn, flow rate 1BV/h. The purified saponin was collected in 70% ethanol eluting solvent. The saponin elution ratio and purity were 89.8% and 239.7%, respectively.The quality standard of Radix Polygalae(Yuanzhi) is absence of a quantitative marker at present. In order to provide quantitative marker, components of Radix Polygalae(Yuanzhi) were studied. Using silica gel column chromatography and preparative HPLC, polygali a ccid(2B,3B-dihydroxy-23 a ,27B-dicarboxyl-13-alkeneoleanane) was isolated from saponin. The structure of polygalic acid was elucidated by UV, HR-MS, 1H-NMR, 13C-NMR, DEPT. it was first isolated from Radix Polygalae(Yuanzhi).Using polygalic acid as a standard substance, quality standard of Radix Polygalae(Yuanzhi) was studied. To establish RP-HPLC method for the determination of content of polygalic acid, diamonds C18 column was used as the stationary phase, the mobile phrase was methanol-water-phosphoric acid(70:30:0.05), detective wavelength was 210nm. The method was accurate and quick, the average recovery was 100.76% with RSD of 2.8%. It can be used for the quality standard of Radix Polygalae(Yuanzhi), which offers the criterion of Radix Polygalae(Yuanzhi) a good foundation.In order to control the quality of Radix Polygalae(Yuanzhi) comprehensively, HPLC fingerprinting of Radix Polygalae(Yuanzhi) was studied. The chromatographic conditions were as follows: 0 min 45% methanol, 30 min 65% methanol and 80 min 45% methanol with gradient elution. The method was proven to be precise, stability and gopd reproducibility, common peaks' area and the ratio of reservation time are all coincident with the request of fingerprinting technology. Radix Ophiopogonis (Mai dong) is a confusable variety of Radix Polygalae(Yuanzhi), Comparing the fingerprinting of them, the ratio of peak superposition is 13.7%, and comparability is 0.072. This method can be used to differentiate conveniently Radix Polygalae(Yuanzhi) and Radix Ophiopogonis(Maidong).The result indicated that fingerprinting is reliable and provides a reference standard for the RadixPolygalae(Yuanzhi).