Study On Mobilized Effects Of Ginseng Polysacchride On Peripheral Blood Hematopoietic Stem Cell

Polysacchride plays a very important regulating role in proliferation and differentiation of cells. Especially the study of polysaccharide in plants has attracted more and more attention of researchers . Ginseng polysaccharide (GPS) is one of the main pharmcologic components extracted from Panax ginseng, Our previous researches have showed that GPS can exactly stimulate the proliferation and differentiation of hematopoietic stem cells and hematopoietic progenitor cells. Peripheral blood stem cell transplantation(PBSCT) is widely applied for clinic assistant treament for tumors , blood diseases and gene therapy in which blood stem cells are used as target cells and so on. However, in normal phsiological conditions, there are only a few of hematopoietic stem/progenitor cells in peripheral blood, which can't meet the needs of PBSCT. So it is an important link of the technique of PBSCT to mobilize adequate hematopoietic stem/progenitor cells into peripheral blood from bone marrow, one key point of which is how to find a safe and effective hematopoietic stem cell mobilizer. The current hematopoietic stem cell mobilizers unavoidably have certain side effects in different ways and some of which are even of severe toxicity. If we can seek out a high-effective and safe mobilizer from polysaccharide of herbs, basic researches and clinical applications of PBSCT can be greatly promoted. In the first part of our study, we used modern techniques of hematology, such as cell culture in vitro , immunocytochemistry (ICC), flow cytometry (FCM) to study the mobilizable effects of GPS on murine peripheral blood stem cells, and to provide academic and experimental evidences for effective and safe mobilizer of hematopoietic stem cell from the treasure of Chinese herbs.Experimental methods: the mice are divided into four groups: saline group (control group) , G-CSF group, GPS group and G-CSF+GPS group. These groups were respectively injected saline , G-CSF , GPS, G-CSF+GPS into mice through one main vein of their tails, then we collect the peripheralblood of each group, and count the number of WBC, MNC, the percentage of CD34+cell in peripheral blood, culture CFU-Mix in vitro at different times regularly.Our experimental results indicate that the number of white blood cell (WBC), mononuclear cell (MNC) in peripheral blood achieved the peak while continued injecting G-CSF into mice intravenously once each day for 5 days and increased at 2h, achieved the peak at 4h, and then returned to normal level at 8h while intravenously injected GPS (1~8mg/kg) into mice. When the dosage of GPS is 2mg/kg, the counts of WBC and MNC reached the maxium, both of which are 4 times of the control group, the percentage of CD34+ in peripheral blood after the mobilization by GPS is obviously higher than that of the control group; The effect mobilized by combination of GPS and G-CSF is much better than that mobilized by single admistration of GPS or G-CSF.Above all, GPS has an obvious mobilizing effect on murine peripheral blood stem cells and has no side effect and toxicity. So it is a great prospective mobilizer. The effect mobilized by combination of GPS and G-CSF is much better than that mobilized by single admistration of GPS or G-CSF. It suggests that we can consider combinating mobilizers to mobilize PBSC in the future practice.