| Skin transplantation is the key to treatment of deep burned wound and skin defect caused by other factors. At present, the main skin sources needed for skin transplantation are from autogenous, allogenous, heterogenous and artificial skin.Autogenous skin is from patients themselves and can be survived perpetually without rejection after transplantation, so it is the best skin graft. However, as for patients with extensive burn, there is greater demand for skin graft than the supply of autogenous skin. In addition,the donor site of skin graft for patients with skin defect is becoming problems. Allogenous skin is from other persons, usually corpse. Because of the difference of histocompatibility antigens between donor and recipient, rejection will occurs in about 3 weeks after tranplantation. Heterogenous skin has shorter surviving time than allogenous skin and both also have a possible problemof carrying virus.Artificial skin is an old topic and has been increasingly focused especially when the tissue engineering has recently developed. Stem cell gives new hope to the tissue engineered skin as seed cell. It resolves the problems of skin resources and immune rejection, thereby it is significant in burn wound healing and repairs. The mesenchymal stem cell (MSC) especially from bone marrow is easily obtained and becoming more and more interesting for investigators.MSC is a population of cells with multipotency, which can be differentiated into osteoblast, chondroblast, tendon cell, adipocyte, bone marrow stromal cell, vascular endothelial cell and neurocyte-like cell. MSC exists in many tissues, such as bone marrow, periosteum, thymus, skin, fat, muscles, and so on. Among those tissue-derived MSC, bone marrow-derived MSC is the easiest to isolate and purify, and has incredible capability of multiplying in vitro, which can meet the clinical requirement with a little of bone marrow, so it will be an important kind of seed cells in tissue engineering. Researchers have in depth investigated the multipotency of MSC since Friedenstein and his colleagues found a method to isolate MSC from bone marrow in the middle of 1970's. However, our understanding of MSC is still little. Now there are many methods ofisolating MSC from bone marrow, for example, centrifuging MSC and purifying them according to their adhesiveness, negative and positive enrichment using the surface markers of MSC. Presently the common used method of culturing MSC is the modified one established by Friendenstein. Cells obtained with the isolation protocol established by Professor Verfaillie seem more pluripotent than those obtained with the common used protocol. Growth factors, such as platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) can be used to induce MSC's differentiation. Recent findings indicate that adult bone marrow also contains cells that can in vivo differentiate into additional mature, non-hematopoietic cells of multiple tissues including epithelial cells of the liver, kidney, lung, skin, gastrointestinal, and myocytes of heart and skeletal muscle.Though there are a lot of reports on MSC's pluripotency, most of the studies were performed in vivo in animals and humans. There are only a small number of studies performed in vitro to demonstrate the ability of MSC to differentiate into osteoblast, chondroblast, adipocyte and neurocyte. Up to now there is no report on MSC differentiating into epithelial cells in vitro. With regarding to the pluripotency of MSC and the reports on their differentiation into epithelial cells of many tissues in vivo, we explored theconditions for MSC differentiating into epidermal -like epithelial cells in vitro.Aims: to isolate, culture, and multiply MSCs from bone marrow aspiration, then induce MSC with conditioned media and detect the expression of epidermal-like epithelial cell markers to explore the possibility of its differentiating into epidermal-like epithelial cell and to found the basis of future study.Materials and Methods1. Isolation, Culture and mul...
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