| Toxoplasma gondii is considered as an important pathogen of human ocular disease. Recent study has proved that the infection of ocular toxoplasma is increasing. Retinochoroidal focus maybe comes from congenital or acquired infection, which occurs at the acute or chronic stage of the infection. In general, the acquired toxoplasma infection in healthy adults is sub-clinical type with different forms, clinical practice shows that many ocular diseases are linked with toxoplasma infection.Most diagnosis of toxoplasma retinochoroiditis is acquired by ocular examination combined with serology test. To some nontypical cases, testing an abnormal toxoplasma antibody reaction in ocular liquid, separating the pathogen or through the pathological way can build the diagnosis of ocular toxoplasma infection. Tox-ELISA is the usual clinical serology method to test the specific toxoplasma antibody at present. Tox-PCR is a recently developed specific diagnostic technique by amplifying the Tox-DNA to test the toxoplasma infection rapidly.Objectives:1)To learn the condition of ocular toxoplasma infection by analyzing clinical cases with ocular disease and mice models, discuss the relation between ocular disease and toxoplasma infection;2)To build animal models of mice acute toxoplasma infection, and observe their pathological changes;3)To provide a convenient and rapid clinical diagnostic techniquefor toxoplasma infection.Methods:l)We studied 120 inpatients and 150 healthy adults. The classifications of ocular diseases: 80 endogenous uveitis, 30 central exudative chorioretinitis, 6 congenital choroidal defect and 4 exudative retinal detachment. The control group composed of 90 college students and 60 farm workers, 17 ocular patients and 76 healthy adults had the history of contacting cat or dog etc. We analyzed the blood preparations of 120 ocular patients and 150 healthy adults by Tox-IgM-ELISA and Tox-PCR. Data were analyzed with x 2test.2)We injected the pured live toxoplasma RH into the abdominal cavity of 30 healthy adult mice(Kunming, half male and half female) in sterile condition, killed them 3 weeks later and extracted both eyes. One eye was performed Tox-PCR test, the other was fixed with 10% Formaldehyde, paraffin section and HE stain. Observed the structural changes of retina and vitreous with light microscope.Results:The sero Tox-IgM test was positive in 11.67% (14/120) patients among 120 cases with ocular disease, 4% (6/150) in the'control group, the difference was significant. (x2=4.65, P<0.05). 26.67% (8/30) positive rate in patients with central exudative chorioretinitis, 8.33% (6/80) positive in patients with uveitis, the difference was significant x 2=5.59,P<0.05). The serology Tox-IgM test was positive in 2.22% (2/90) college students and 6.67% (4/60) workers. The difference was not significant x 2=0.86, />0.05).The results of sero Tox-PCR test: 14 Tox-IgM positive cases with ocular disease were all positive in PCR test, 5 cases were PCR positive in 6 Tox-IgM positive cases among the healthy control group. The difference was significant x 2=5.86,P<0.02).The Tox-PCR test was positive in the experimental mice ocular tissue. The pathological section found that there was no necrosing change in every layer of retina and choroid. The pigment epithelium layer detached from the inner layer of retina, and there was amount of inflammatory cellular infiltration in each layer. Lots of new vascularformed in the retinal nerve fiber layer and some of them stretched into the vitreous body thus induced the inflammation and proliferation, and we could see red cells and inflammatory cells in the vitreous.Conclusion:1)The rate of the toxoplasma infection in this group is much higher than that in healthy adults, and the Tox infection rate in central exudative chorioretinitis is higher than uveitis. It is proved that some ocular diseases are connected with toxoplasma infection, and we should pay much attention to it in clinic.2)The sero Tox-IgM-ELISA is a simple and sen...
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