Preparation Of Gelatin-Chitosan Crosslinked Film And Experimental Study On Its Effect Of Adhesive Prevention

[Objective] To prepare gelatin-chitosan crosslinked film(GCCF) and to study its hemostatic and adhesion-prevented properties. [Methods] GCCF was prepared by casting the mixed solution of dialysed chitosan and gelatin after crosslinking with glutaraldehyde in freeze dryer. Bleeding times were measured for bilateral back incisions(5 incisions on each side) in 4 New Zealand white rabbits. Using a randomized, blinded experimental design, one incision in each animal was treated with GCCF, the other was treated with gauze. After bleeding stopped , bleeding time and hemoglobin amount in films or gauzes was measured to determine the hemostatic effect. Bilateral craniotomies were performed at calveria in 16 adult rabbits. Dura was removed. A piece of GCCF was put in one randomized side and the other side served as a control. The post-craniotomy scar formation was evaluated grossly and histologically on weeks 2, 4, 8, 12.[Results] GCCF adhered to incision well. Bleeding times and hemoglobin amounts in GCCF group were superior to those in gauze group (P<0. 01). In the treated sides, no adhesion was observed, and the space between scar tissue and Pia mater or encephalon was well preserved. In the control sides, adhesion of Pia mater or encephalon to the scar tissue occurred to the entire exposed surface. The difference was significant (P<0.05) . [Conclusion] Chitosan film was effective in hemostasis for Incision. GCCF promises to be effective in preventing adhesion of scar tissure.