| Cellular compatibility of ethylenediamine modified poly(DL -lactic acid)(EMPLA ) and acellular porcine dermal matrix(APDM) were studied through cell culturing method. Osteoblasts were cultured on the surface of PLA and EMPLA, and cell morphology, cell proliferation, cell adhesion and cell function were studied, with glass as control. Fibroblasts and endothelial cells were cultured on the surface of acellular porcine dermal matrix (APDM), and cell histology, cell proliferation were studied. To screen out tissue engineering materials which have good biocompatibility, the biocompatibility of EMPLA and PLA has been evaluated respectively. The main works and conclusions are included as follows :(1) Cell culture : osteoblasts obtained and cultured from crania of neonatal Wistar rats by tissue blocks method ; fibroblasts(NIH/3T3 ) and endothelial cells (ECV-304 ) were cultured.(2) Compatibility of EMPLA with osteoblasts:a) Cell morphology was observed and recorded with discrepancy microscope. The results showed that : the osteoblasts cultured on EMPLA spread wider than those on PLA, and much more cells were confluent on EMPLA compared to those on PLA and glass. The results indicated that EMPLA has better compatibility with osteoblasts than PLA and glass in cell morphology. b) Cell proliferation was determined by MTT assay at 2d,4d,6d and 8d , and a growth curve was drawn . The results showed that : cell growth activity and the shape of the growth curves are different for the different substrates. The cell growth activity of osteoblasts on EMPLA is significant higher than on PLA and glass, and on PLA the cell growth activity is significant higher than on glass. The results indicated that EMPLA has better compatibility with osteoblasts than PLA and glass in cell growth.c) Cell adhesive force of single osteoblast was studied with micropipette aspiration technique at 15min and 24h. The results showed that : osteoblasts on EMPLA at 15min and 24h had better adhesive force than on PLA and glass , and on PLA had better adhesive force than on glass at 15min and 24h. The results indicated that EMPLA has better compatibility with osteoblasts than PLA and glass in cell adhension.d) Alkaline phosphatase (ALP) activity of osteoblasts was determined by enzyme dynamical method at 2d,4d and 6d . The results showed that : From 2d to 6d , the ALP activity of osteoblasts on the materials increased. At 2d, there was no difference between the materials. At 6d, osteoblasts on EMPLA had higher ALP activity than on PLA and glass. At all times, there was no difference between PLA and glass. The results indicated that EMPLA has better compatibility with osteoblasts than PLA and glass in cell function.All the results summarized about cell morphology, cell proliferation, cell adhesion and cell function , it indicated that much better biocompatibility of EMPLA with osteoblasts than PLA and glass , suggesting the potentially wide applications of EMPLA in biomedical area, especially in tissue engineering.(3) Compatibility of APDM to fibroblasts(3T3) and endothelial cells(VEC):a) VEC were cultured on APDM for 1 week , then made a histological observation. The results showed that : VEC had formed a monolayer, in some places ,such as pore even had formed 2 to 3 layer .b) Cell proliferation of 3T3 and VEC were determined by MTT assay at 1d,2d and 4d, and a growth curve was drawn . The results showed that : 3T3 and VEC grown much rapidly on APDM . At 1d, the cell activity of 3T3 and VEC on APDM were significant higher(P﹤0.01) than on control. Namely, the cell activity of 3T3 on APDM was 7 times more than its control, the cell activity of VEC on APDM was about 4 times more than its control. At 4d, cells on control grown faster than on APDM, but there was also a significant difference between APDM and control. All the results about cell histology, cell proliferation were summarized ,it indicated the good biocompatibility of APDM with 3T3 and VEC, suggesting APDM potentially wide applications of EMPLA in biomedica...
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