The Expression Of Resistin And Adiponectin In Fat Tissue Of Rat Model With Type 2 Diabetes Mellitus

Objective: To characterize the relationship between resistin and adiponectin in obesity and type 2 diabetes mellitus by examining resistin and adiponectin gene expression in visceral fat of obese rats and rats with type 2 diabetes mellitus.Methods: (1). Female Wistar rats were fed with a diet enriched with triglyceride (37. 5%, w/w) and cholesterol (1%, w/w), and after nine weeks on the diet streptozotincin (STZ, 25mg/kg)was injected intraperitoneally to induce insulin resistance and hyperglycemia. <2) .The resistin and adiponectin mRNA expression level in adipose tissue of 23 rats with type 2 diabetes mellitus, 10 obesity rats and 10 control rats were assayed by semiquantitative reverse transcription-polymerase chain reaction. Results: (1) . In STZ-treated rats, the body mass and serum triglyceride was markedly increased;fast plasma glucose lever were normal, but postprandial plasma glucose lever were markedly increased;glucose tolerance were impaired,-fast plasma insulin concentration were higher than controls. (2) .The adiponectin mRNA expression level in omental adipose was significantly decreased in obesity rats and rats with type 2 diabetes mellitus than non-diabetes control rats(P<0.05). On the contrary, resistin mRNA level significantly increased in obesity and type 2 diabetes mellitus.Conclusion: (1).This novel rat model simulates human type 2 diabetes in obesity, hyperglycemia, hypertriglyceridemia and insulin resistance. It may provide a particular advantage for investigating type 2 diabetes and diabetic complications. (2).Our data suggest that adiponectin down-regulation and resistin up-regulation may play an important role in insulin resistance associated with obesity and diabetes mellitus. The level of adiponectin and resistin mRNA in visceral adipose tissue seems to be an important parameter of insulin resistance.