Experimental Study Of The Antiatherogentic Effect Of Buqihuoxuehuatan Decotion In Rabbits

Objective:To investigate the antiatherogenetic activity of Chinese medicine Buqihuoxuehuatan decoction in male rabbit fed an atherogenic diet and the effect of the total cholesterol, triglycerides, low-density lipoprotein-cholesterol apolipoprotein B high- density lipoprotein- cholesterol and apolipoprotein Al level in serum, and investigate the nitric oxide and endothlin-1 level in plasma.Materials and Methods:An atherosclerotic rabbit model was established by feeding high cholesterol diet supplemented by bovine serum albumin injection bolus. The rabbits were randomly divided into three groups: the model group, Buqihuoxuehuatan Decoction treated group and nifedipine treated group. After fed for twelve weeks, all animals were killed to take blood sample through heart to measure , and to take aortic to determine the percentage of aortic lesions covering the intimal surface and to observe structure with light microscope ,and coronary arteria sample to observe structure with light microscope.Results:1. Feeding high cholesterol diet supplemented by bovine serum albumin injection bolus can establish an atherosclerotic rabbit model.2. Buqihuoxuehuatan Decoction can decrease the aortic atherosclerosis (P < 0.05 ) .3. Buqihuoxuehuatan Decoction can increase the serum high-density lipoprotein-cholesterol apolipoprotein A1 and plasma nitric oxide, and decrease the serum total cholesterol, low-density lipoprotein-cholesterol, apolipoprotein B and the plasma endothlin-1 (P<0.05, P<0.01) .Conclusion:Buqihuoxuehuatan Decoction had antiatherogenic effects and its mechanism might associate with modifying the serum lipid , preserving endothelial function and maintaining the balance of NO and ET-1.