| [Objective]: To explore the protective effect of QRHX prescription based on the main principle of Qing Re Huo Xue on cerebral ischemia (CI) injury.[Methods]: The rat models with CI were prepared and randomly devided into normal group, sham—operated group, CI group, treatment group with different dosage of QRHX prescription, and tetramethylpyrazine control group. The changes in the following parameters, including the contents of interleukin—1β(IL—1β), Intercellular adhesive molecule(ICAM—1), Polymorphonuclear leukocyte (PMNL), excitatory amino acid(EAA) and water in brain tissue were observed, with methods of ELISA and immunohistochemistry and HPLC respectively at 3h,6h,12h,24h after operation. Meanwhile, the different pathologic changes were observed by light microscope and the neuron number in the hippocampus CA1 was counted at those points respectively.[Results]: QRHX prescription was able to decrease the contents of IL-1β, ICAM-1, and EAA in brain tissue obviously (P<0.05).The difference was significant as compared with QRHX high dose group and tetramethylpyrazine control group(p<0.05). The neuronal degeneration and lightly neucrosis and glue hyperplasia occurred in hippocampus areas of the rats pretreated by drugs, but the damage was more severe in the model group. The number statistics difference of neuron in CA1 area between themodel group,Sham-operated group and drug-pretreated was significant (p<0.05).[Conclusions]: The results suggest an important role for IL-1β,ICAM-1 mediated PMNL adhesion in the evolution of brain injury following CI. Downregulating the expression levels of IL-1β and ICAM-1 protein, and inhibiting the accumulation of PMNL may ascribe to the protective effects of QRHX prescription on inflammatory brain injury following CI. |
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