The Experimental Study Of G-CSF Expression And Promoting Cell Proliferation In Human Solid Carcinomas

Objective: In order to provide the experimental evidence for secure application of rhG-CSF, G-CSF expression and promoting cell proliferation in 7 kinds of human solid carcinoma cell lines were investigated. Methods: The expression of G-CSF from these cell lines was detected by Enzyme-Linked Immunoabsorbent Assay Kit. The G-CSF production of G-CSF positive cell line was further investigated at different time point. rhG-CSF promoting cell proliferation was studied by cell count, MTT and colony formation assay. Results: 1. Cell line T-24 produced large amount of G-CSF(>2000ng/ml), which increased with time going. At the same time, G-CSF expression was not observed in other 6 cell lines; 2. rhG-CSF promoted cell line KB proliferation in vitro. After rhG-CSF administration at a concentration of 100ng/ml, the maximum of cell count, OD value, proliferative rate and colony number of cell line KB reached (10.6±0.1) × 104/ml, 2.343 ± 0.105, 35.2% and 257±24.8/well respectively, which had significant difference compared with control group (P<0.05). No proliferative effect was observed in other 6 cell lines; 3. Cell line GLC surviving and proliferation was observed in medium containing 5% calf bovine serum. Conclusions: 1. Cell line T-24 produced large amount of G-CSF, which increased with time going; 2. Exogenous rhG-CSF promoted KB cellproliferation in vitro and reached its maximal proliferative effect at a concentration of 100ng/ml; 3. Cell line GLC can survive and proliferate in medium containing 5% calf bovine serum probably by secreting some growth factors; 4. For patients with G-CSF receptor positive carcinoma, high dose G-CSF shouldn't be administrated without combined treatment of radiotherapy or chemotherapy.