Study On Neuroprotection Ingredients Of Erigeron Breviscapus

Erigeron breviscapus, family Compositae, was used to treat sequelae of apoplexy by the minority nationality (e.g. the Miao) all through the ages. In recent years, researches widened it use in ophthalmic disease and indicated that its mechanism might be neuroprotection. This dissertation reports the discoveries of neuroprotection ingredients in E. breviscapus and covers several fields involving ethnomedicine, ophthalmology. TCM-chemistry, pharmacology and document-analysis.1. Study on ethnic useA survey of this herb's historic documents, source, distribution, indication, irregular names, ethnic experiences and artificial culture trends was summarized in the paper.2. Analysis of ophthalmic references and chemical constituents in several clinic preparationsImportant finds were achieved after summing up ophthalmic progress and comparing the difference of HERBA EREGERONTIS, INJECTIO ERIGERONTIS and TABELLAE BREVISCAPINI by HPLC. The results are: (1) INJECTIO ERIGERONTIS and TABELLAE BREVISCAPINI have completely different compounds and completely identical indications; (2) Breviscapine and caffeoyl conjugates are both active compounds in E. breviscapus for curing brain, heart and eye disease. Then, the thesis developed a new research pattern named "Clinic-Chemistry". In addition, the relativity between ophthalmic treatment and heart treatment are approached.3. Study on neuroprotection effective fraction in E. breviscapusThe fraction C and G were picked out as neuroprotection substance form 7 samples of E. breviscapus by cultured immature-rat retina nerve-cell using MTT colorimetric analysis, thereafter, an optimal technological process for the neuroprotection effective fraction was developed.4. Study on chemical constituents of the effective fractionSilica gel column chromatography and preparative HPLC were used. Five compounds were isolated and 4 of them were identified as scopoletin(I), caffeic acid (II), 3-0-caffeoyl quinic acid (III) and scutellarin (IV) by spectrum (UV, IR, MS, 'H-NMR and 13C-NMR) and document data. Compound III was isolated from E. breviscapus for the first time.