| Object: With food component and lifestyle improving, the incidence of fatty liver in our country increase year by year. Incidence of fatty liver disease with type 2 diabetes mellitus and obesity is 50%. Recently with obese children increasing, the incidence of fatty liver disease tends to young, in the 40 cases of simple obesity children regular average age 9 years olds, fatty liver by ultrasonography is 38%, but fatty liver definite risk factor and pathogenesis have not been clear so far. Recently, domestic and international research show that nonalcoholic fatty liver has close relationship between serious obesity especially abdominal type obesity, dyslipidemia and insulin resistance syndrome have close correlation, and leptin have close relation with obesity, lipodystrophy and insulin resistance also. Some research show that serum leptin level increase in nonalcoholic fatty liver patients. The high calorie food can cause obesity and insulin resistance, seriously, which can lead to diabetes. SD female rat fed with special diet were led to insulin resistance.Little dosage streptozotion (STZ) was injectedwhich brought out hyperglycemia. so, type 2 diabetes rat model was established. This model has characteristics of hyperglycerolemia, hyperglycemia and hyperinsulinism, which was similar to human type 2 diabetes mellitus. On this foundation, we discussed influence of different calorie food on obesity, dyslipidemia, insulin resistance; the relation between leptin, insulin resistance and lipodystrophy; correlation between leptin and fatty liver; correlation between fatty liver, insulin resistance and dyslipidemia. Method: The rats were randomly assighned into four groups: one was normal control rats group (group A) others was diabetic rats group (group B,C,D). Group A was fed with normal diet. Group B was fed with high-fat diet (the calorie that fat offered was 70%, the calorie that carbohydrate offered was 13% for the calorie that 17% and protein offered),Group C was fed high-sucrose diet ( the calorie that carbohydrate offered was 70%, the calorie that fat offered was 17%, the calorie that protein offered was 13%) ,Group D was fed high-sucrose-high-fat diet ( the calorie that carbohydrate offered was 40%, the calorie that fat offered was 47%, the calorie that protein offered was 13%) . For 4 weeks, insulin resistance was brought out, then little dosage STZ (25 mg/kg), was injected in abdominalcavity, so pancreases was impaired partially, which bring out pancreases secrete obstacle. It was not overcome insulin resistance and led to hyperglycemia. Whole experiment last26 weeks, Samples of blood were obtained at 4, 8, 26 weeks to measured fasting blood glucose ( FBG ) , fasting serum insulin ( FIN ) , serum triglyceride ( TG ) , serum cholesterol ( TC ). When the experiment of 26th was finished, ALT, AST and serum leptin was measured. Liver tissue was observed by naked eyes and microscope. Liver tissue was stained with HE staining and fat staining. Ultrastructural chang of liver tissue was observed by electronic microscope. According to the fatty staining, the fatty liver degree was evaluated. Correlation between leptin and insulin sensitive index (ISI); correlation between leptin and TG, TC, ISI, fatty liver degree and ALT respectively were compared. Result:Diabetic groups rats with special diet fed 4 weeks later, rats body weight was significantly heavier than control group (p<0.05), but there was no significant difference between diabetic groups (p>0.05). TG, TC increased compared with control group (p<0.01, p< 0.01), each of diabetic groups did not have significance difference (p>0.05). Compared FBG of diabetic group with that of control group was no significant difference (p>0.05), each experiment group without significance difference (p>0.05). ISI was lower than control group (p< 0.01), and there was no significant difference in each experiment group (p>0.05), above data showed special food can lead to insulinresistance 4 weeks later. After little dosage STZ wa...
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