Study On TNF α And IL-6 Secreted By Stimulated Peripheral Blood Mononuclear Cells By Estradiol In Women With Systemic Lupus Erythematosus

Objective:Systemic lupus erythematosus (SLE) is an autoimmune disease involved heredity, sex hormone, enviroment, immunologic reaction and so on. It always invades many organs in body and has a 10~13:1 F: M ratio. The peak incidence rate appears in child-bearing period women who get higher activity when they are pregnant. It's well known that sex hormones especially estrogen play an important role on episode of SLE. More study in this field should be done for its unclear pathogenesis. The sera estradiol (?) level in gestational period is much higher than that of in normal time. Our experiment is going to study the peripheral blood mononuclear cells (PBMC) which will secret different level of THF a and IL-6 with and without estradiol whose concentration is similar to be in the metaphase of pregnancy. We try to explain the reason for the illness's more serious state under the condition of high estrogen though our work, which could establish new pathway to the truth of the disease.Methods:Sera ? were measured by radioimmunoassay in patients with SLE and healthy controls. PBMC of the two groups were collected and then cultured for 24h with or without ? in the sterile incubator under the condition of 3 7 C and 5%CO2. The supernatants were aspirated and frozen at -80癈 until they were tested with commercial kits by enzyme-linked immunosorbant assay (ELISA). Values were given as average and standard deviation (X+S). Significance of results was measured by the t test. Correlations between results were measured by linearcorrelation or Spearman rank correlation wherein r=1.0 is perfect correlation, r=0.0 is no correlation, and r=-1.0 is perfect inverse correlation. A p value of 0.05 was considered significant.Results:1. The E2 level in serum of patients with SLE was higher than that of healthy controls. (0.020.05). In the patients, TNF a level with E2 was lower than that of without E2. (0.010.05).5. The IL-6 level without E2 in controls was higher than that of with E2. (0.020.05)8. The sera E2 level had no correlation with DAIS in patients. (P>0.05)9. There is no statistical significant between TNF a and IL-6 level without E2 in patients. (p>0.05).Conclusion:1. The sera E2 level in serum of patients with SLE was higher than that ofhealthy controls, but the E2 level had no correlation with DAIS in patients. It suggests that the SLE patients could have higher E2 level which couldn't affect disease activity directly.2. Both the TNF a and IL-6 level secreted from PBMC without E2 in patients were higher man those of in controls while they both have positively correlation with DAIS but have no correlation with E2. These results suggest that the TNF a and IL-6 level secreted from PBMC in normal condition related closely with onset of SLE, and they can be regarded as a index to disease activity. However, the sera E2 in normal time could not play an important role on them.3. The TNF a level after E2 stimulating was much lower than that of without E2 in patients, but had no severely different in controls. We suggest the E2 in high concentration may inhibit TNF a secreting by PBMC of SLE which distinguish from the normal.