| IntroductionLung cancer is a common , frequently - occuring disease in our country , at present, the study about the relativity of oncogene , tumor suppressor gene and lung cancer has become a hot spot in lung cancer tumor molecular biology and genetics in our country and abroad. p73 gene is a kind of tumor suppressor gene, a member of p53 families, first found by Kaghad in 1997 , located in 1p36. 2 -1p36. 3. p73 protein is similar to p53 protein on structure and function, so some scientists think it as a tumor suppressor gene, but, the present study consider : the level of expression of p73 is different in all kinds of tumors, p73 has become a hot spot in tumor molecular biology and genetics. Otherwise, the physiological and other functions of p73 still need a further study. Scientists now have still discuss about the status and functions of p73 gene in the occurrence and development of the tumors.In recent fifty years, morbidity and mortality rates of lung cancer have risen, become a fast - increasing carcinoma. As a main disease all over the world, lung cancer has done harms to human health and lives. The study examines and analyzes the expression of p73 protein in primary Non - Small Cell Lung Cancer through immunity histochem-istry method and discuss its functions in the occurence, developmentand metastasis of lung cancer.Materials and methods85 cases of non - small cell lung cancer samples and 32 cases of normal lung tissue were obtained from The First Affiliated Hospital of Chinese Medical University from March to June,2000. The maximal age is 80 years old , the minimal age is 30 years old , the mean age is 60.7 +2.6 years old; male; 48 cases, female; 37 cases, it includes 42 cases quamous cell carcinoma and 43 cases adenocarcinomal. The chemotherapy and radiotherapy were not used before surgical operation in all cases, with the systimic lympth node expurgation in the operation. It is approved by pathology post - operation. The TNM - stages of tumor are difmed by WHO in 1981 and UICC standard in 1997.1. slice preparing: general olefin embed, chop in 4 micron series of slices, every example is chopped in three slices prepared for HE and p73 dyeing, another one is prepared for negative comparison. All the slices are dealed with APES liquid.2. p73 dyeing: generally, slice is missed out wax to hydrate, by antigen repairing, drop in non - antigen anminal serum, incubate 5 minutes in normal room temperature ,wash up in PBS liquid. All the steps of s - p immunohistochemistry were done by the introduction of s-p box-, joining up first and second antibodies, DAB, dyeing with haematoxylin again, slice is covered by general clarity gum.3. result determinant: p73 protein is located in nucleolus, different brown reaction , brown - dyeing cells excess 10% is regarded as masculine result in high power lens, The results were analyzed by x , P <0.05 is significant.ResultsThere were 56 cases of positive expression and all these occupied 65.9% of all the 85 cases. The negative rate were 34. 1% ;In the 43 cases of adenocarcinomas, 33 cases were positive(76. 7% ) ; 23 cases were positive in 42 cases of lung squamous cell carcinoma(54. 8% ) . The statistical analysis of x indicates that the expression of p73 in non - small cell lung cancer is higher than normal lung tissue (P < 0. 01), the expression of p73 in adenocarcinoma is higher evidently than squamous cell carcinoma (P < 0. 05) , evidently not related with tissue differentiation (P >0.05). Positive rate of p73 in I and II stages is 55. 3% (26/47) , the positive rate in IIIA is 78. 9% (30/38); The expression of p73 is becoming increasing with TNM increasing in NSCLC. 85 cases NSCLC have 54 cases Lympth node metastasis ( N1-2) and 31 non - Lympth node metastasis ( N0) . The positive expression rate of p73 in N1-2is 74. 1% (40/54) , the positive expression rate in N0 is 51. 6% (16/31 ), evidently different ( P < 0. 05 ). The positive expression rate of p73 in male and female patients is respective 70. 8% , 59. 4% ; The positive...
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