| IntroductionEndometrial carcinoma is one of the three most frequent malignancy in female genital tract, which is 7% of the all female malignancy and 20% -30% of the malignancy in female genital organs. Recently , its incidence is gradually elevated on world scale. Endometrial carcinoma becomes a major disease that threaten female lives. Thus, studies on etiology, methods of early diagnosis and effective treatment for endometrial carcinoma become focus to be investigated. With the rapid development of molecular biology technology, oncogene, anti -oncogene and related substance abnormality are believed as basical cause of normal tissues carcinogenesis and series changes of biological patterns. Epidemiological studies have documented a 40 -50% reduction in the incidence of colon cancer in individuals taking non - ster-oidal anti - inflammatory drugs ( NSAIDs). The reason relates to the inhibition of NSAIDs to cyclooxygenase ( COX ). COX is a membran conjugated protein and is the rate - limiting enzyme in the synthesis of prostaglandins. Presently, it is known that human cell contains at least two isoforms of COX genes coding, COX - 1 and COX -2. COX - 2 is an inducible immediate - early gene that is upregulated by cyto-kines, growth factors, and mitogens. More and more studies have demonstrated that COX -2 expression is aberrantly increase in varioushuman epithelial cancers, including those of cocorectal, esophageal, lung, gastric, cervix and bladder origin, but there still have no reports about COX - 2 expression in endometrial carcinoma and relationship between COX - 2 expression and clinical pathological characters. In the present study semi - quantity reverse transcription polymerase chain reaction ( RT - PCR) was used to detect the expression of COX -2 mRNA in 32 endometrial carcinoma, 10 hyperplasia and 10 normal endometriums to determine the relationship between COX ?2 and carcinogensis and development of endometrial carcinoma.Material and methodSpecimen: A total of 52 fresh endometrial tissues were collected at the department of gynecologic in the First and Second Clinical Hospital of China Medical University from Mar 2000 to Mar 2002 after surgical operation. They were divided into 3 groups.- 1. 32 endometrial carcinoma; 2. 10 hyperplasia endometriums; 3. 10 normal endometriums. All the case are primal patiens, median age was 53. 7. Part specimen were fixed in formalin, perform HE stain, to confirm pathological diagnosis, histology type and histology grade. The others were snap - frozen in liquid nitrogen then stored at -70t for RT - PCR. The histological type of all the 32 endometrial carcinoma tissues were adenocarcinomas (n = 22 ) , adenoacanthomas ( n = 7 ) and clear cell carcinomas ( n = 3 ) . FIGO surgical staging system were stage I ( n = 18 ) , stage H ( n = 9 ) and stage HI (n = 5 ). The histology grade wereGi(n = 14), G2(n = 10) and G3(n =8). There were 12 With deep myomertrial invasion, 5 With lymph node metastasis and 4 with abdominal cavity fluid cytology positive in the 32 endometrial carcinoma tissues.RNA extraction and RT - PCR: Total cellular RNA was extracted by the acid guanidium - phenol - choroform method and cDNA was synthesized with reverse transcriptase followed by amplification PCR with cDNA cycle kit . The conditions of amplification were as follows: 35cycles of 90s for denaturation at 941, 45s for annealing at 55 C and 90s for extension at 12 C. A final extension at 72 C for 10 min. The primer used were: A 10ul of amplified product was then electrophoresed on a 2% agarose gel and bands were visualized by ethidium bromide staining. 1D KODAK gel image analysis software was used to scan the RT -PCR agarose gel after photographic documentation.Analysis of results: It was positive when both 305bp and intra -control's 690bp bands were found ; it was negative when only intra -controls 690bp band was found . Relative content of COX - 2 = ( COX -2 density/(3 -actin density) + 100Statistical Analysis: statistical analysis was carried out using SPSS. The...
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