| Objective: Endometrial carcinoma is a group of epithelial malignant tumors in the endometrium,Endometrial adenocarcinoma is the most common form of endometrial carcinoma which is one of the three malignant tumors of female genital tract[1].It accounts for 7% of all female malignant tumors,accounting for 20%-30% of female genital tract malignancies.In developed countries and some economically developed cities in China,the incidence of endometrial cancer ranks first in gynecological malignancies.At present,the incidence of the disease is increasing in the world[2],and obviously younger.For the pathogenesis of the disease,the clinical view is "no resistance to estrogen" theory,but has not yet fully clear[3].According to whether relied on estrogen or not,the endometrial carcinoma is divided into two types: estrogen dependent type(type Ⅰ)and estrogen independent type(type Ⅱ).The type Ⅰ is endometrioid adenocarcinoma,which accounts for the majority of endometrial carcinoma.Patients with this disease are usually younger,often associated with obesity,high blood pressure,diabetes,infertility and menopause[4].The type Ⅱ is a rare type of cancer,which is more common in older women with thin body,and the specific cause of the disease has not been found.Cyclooxygenase(COX)-2 is an inducible transcription enzyme,which can not be detected under normal physiological conditions,but is highly expressed in various malignant tumors.In recent years,it has been found that COX-2 is involved in many kinds of tumor cell proliferation,anti apoptosis and angiogenesis,and is closely related to the occurrence,development and prognosis of tumor[5].It is believed that type I endometrial carcinoma is closely related to neuroendocrine.There is only a small amount of endocrine cells in the normal endometrium,but the common endocrine cells in endometrial carcinoma.The significance of these endocrinecells and their relationship with the biological behavior of endometrial cancer is not clear.Chromogranin A(Cg A)is a specific marker of neuroendocrine.In this study,we detected the expression of COX-2 and Cg A in type I endometrial carcinoma and normal endometrium to investigate the role of both in the occurrence and development of endometrial carcinoma,and to explore the relationship between the two in the occurrence and development of endometrial carcinoma.Furthermore,it provides guidance for evaluating the prognosis of endometrial carcinoma and developing new clinical pathway for the treatment of endometrial carcinoma.Methods:1 This experiment selected 52 patients with endometrial cancer surgery in Hebei general hospital from May 2014 to August 2016.The results of their postoperative pathological findings were all endometrioid adenocarcinoma and their specimens were taken as experimental group.The control group was selected for the same period of 22 cases of uterine fibroids,ovarian cysts and other benign lesions underwent total hysterectomy,and the pathological results of them were benign proliferative endometrium.2 This experiment selected 52 patients with endometrial cancer surgery in Hebei general hospital from May 2014 to August 2016.The results of their postoperative pathological findings were all endometrioid adenocarcinoma and their specimens were taken as experimental group.The control group was selected for the same period of 22 cases of uterine fibroids,ovarian cysts and other benign lesions underwent total hysterectomy,and the pathological results of them were benign proliferative endometrium.3 To analyze the relationship between the expression of COX-2 and Cg A protein and the clinicopathological features of endometrioid adenocarcinoma.4 Analyse the data using SPSS 17.0,and it is the statistically significant when P<0.05.5 Using Spearman correlation test to analyze the relationship between the expression of the two proteins in endometrioid adenocarcinoma.Result:1 The positive expression rates of COX-2 in normal endometrium and endometrioid adenocarcinoma were 36.36% and 80.77%,respectively.The positive expression rate of endometrioid adenocarcinoma group was significantly higher than that of normal endometrium group,the difference between the two groups was significant(P<0.01).The expression of COX-2was correlated with the pathological stage,depth of myometrial invasion,histological differentiation,vascular invasion and lymph node metastasis.2 The positive expression rates of Cg A in normal endometrium and endometrioid adenocarcinoma were 18.18 %、 50.00 %,respectively.The positive expression rate of endometrioid adenocarcinoma group was significantly higher than that of normal endometrium group,and the difference between the two groups was statistically significant(P<0.01).The positive rate of Cg A was significantly higher than that in poorly differentiated tissues in endometrioid adenocarcinoma.The positive expression of Cg A in endometrioid adenocarcinoma was not significantly correlated with the pathological stage,histological differentiation,depth of myometrial invasion and lymph node metastasis.3 Expression of both proteins in endometrial adenocarcinoma was positively correlated(r=0.488 P=0.000).Conclusion:1 COX-2 was highly expressed in endometrial adenocarcinoma tissue,and the expression of COX-2 was related to the surgical-pathological stage,the depth of myometrial invasion,the histologic grade,vascular invasion and lymph node metastasis,and it has a certain relationship with the occurrence and development of endometrial cancer.2 Cg A was highly expressed in endometrioid adenocarcinoma,and it had no significant correlation with pathological stage,histological differentiation,depth of myometrial invasion and lymph node metastasis of endometrioid adenocarcinoma.3 There was a positive correlation between the two expression in endometrioid adenocarcinoma,which may have a synergistic effect in theoccurrence and development of endometrial carcinoma. |
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