| IntroductionLumbricus is annelid and is salty taste. Itsnature is rhigosis. Lumbricus can scaveng fever and banlance liver function and it is anti-asthmatic. In clinical lumbricus or its complex prescription is used to therapy bacterium and virus infection and dermic burn that is liable to infection and the effect is very good. Also lumbricus is used for some tumor and immunological diseai.se such as refractory minute lesion ne-phrosis, idiopathic thrombocytopennic purpura ( ITP) and systemic lumpus erythematosus (SLE). So it is thought that this is because lumbricus could adjust immunological function .It is studied that extracts of live lumbricus can increase phagocyte activated rate and the quantity of Fc receptor on the surface of phagocyte. So it can enhance phagocyte immunological function obviously. "912" capsule made of extracts of lumbricus can inhibit some kinds of neoplasm cells, elevate thymus weight and the quantity of thymocytes of noduliferous mouse. In 2002 Lin SQ et al confirmed that QY - 1 extracted from lumbricus can enhance the phagocytic function of macro-phage. It can increase splenic and thymus index of noduliferous mouse. And it can also increase activity of three blood antioxidase -CAT, SOD and GSH - Px. But now because the purification of the extracts of lumbricus is low and its application is directly derived from bactericide and killing tumor cells, the application of lumbricus is limited.Lumbricus peptides(LP) is extracted from live lumbricus in ourlaboratory. It have been confirmed that LP have some biological activity in vitro and its purification is high. In our laboratory it have been studied that LP can elevate the rate of nature killer killing tumor cell and enhance inocytosis of macrophage. On the base of these we continued to explore the influence on immunological function of LP from the two levels of cell and molecule in vivo.Materials and Methods1. Materialslumbricus peptides(LP) , C57BL/6 inbred line mouse, ConA, MTT, actinomycin D, IL - 2 and TNF - a standard control, dimethyl sulfoxide( DMSO) ,Griess reagent, and so on.2. Methods2. 1 Animal grouping and treatingC57BL/6 inbred line mice were distributed to six group at random and that is normal control, LP Img/ml, LP 0. 5mg/ml, LP 0. Img/ml, cytoxan and cytoxan plus LP.2. 2 L929 cell were used as target cell to determine macrophage cellulotoxic effect and the level of tumor necrosis factor alpha. To detect the level of NO, diazo - reaction is used. Splenic lymphocyto proliferating stimulus index is determined by MTT. And the level of IL -2 is detected by mouse splenic cell activated with conavaline A.ResultLP 0.5,0. Img/ml can enhance immunological function of mouse and the latter is more obviously. Compared with control , the macro-phage cellulotoxic percentage and the level of NO and TNF - a secreted by macrophage or splenic cell rise in the group of LP 0. Img/ml. And so is splenic lymphocyte stimulus index and the level of IL -2( P <0.05).Injecting cytoxan made mouse immunological function lower and LP 0. 5mg/ml can antagonise the inhibition of cytoxan.DiscussionLumbricus is convential Chinese medicine . It is used extensive ly. In clinical lumbricus or its complex prescription is used for infectious disease, immunological disease and tumor. So it is thought that lumbricus could adjust immunological function.It is reported that extract of lumbricus can activate macrophage, enhance macrophage activity , increase the quantity of Fc receptor on the surface of macrophage. "912" capsule made from lumbricus can inhibit several kinds of tumor cells, increase the thymus gland weight and the quantity of thymocyte. In 2002, Lin SQ et al reported that QY -1 - an extract of lumbricus can distinctly enhance phagocytic function of macrophage and thymus index of noduliferous mouse. It can also increase three kinds of blood antioxidase - CAT, SOD and GSH -Px.But now the application of lumbricus is limited because its extract is not purifided and the therapy is directly...
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