Studies On Different Protein Expression During The Oocyte Meiosis Maturation

During the rapaidly development of assistant reproduction technology, more and more sterility patients have gotten successfully pregnancy. However, some patients still haven't gotten successfully pregnancy because of some reason, such as PCOS, the risk of OHSS, and immature oocyte during the cycle, etc. So the oocyte in vitro maturation technology is emerging and show its importantly significance to these patients. The molecular mechanism of oocyte maturation become to the hot project in the female reproduction field. Previous research have already proved that oocyte maturation accompany protein synthesization andphosphorylation;dephosphorylation status exchange during the oocyte maturation. StAR (steroidogenic acute regulatory protein), MPF, MAPK, PKC, and cAMP/PKA have already been proved that involved in oocyte maturation, however, there_are still many novel proteins or unkonwn proteins which might take part in this process. In this research, we want to identify several proteins that might regulate oocyte meiosis maturation/ follicular development using the high resolution two-dimensional (2-D) electrophoresis and mass spectrometry. We have divided in four parts to study it.Part one The development and preliminary application of Two-dimensional electrophoresisand Mass spectrometry in ovary proteomeresearchAbstract Proteome analysis is 'the analysis of the PROTEin complement expressed by a genome'. Two-dimensional electrophoresis is now a powerful and widely used method for the analysis of complex protein mixture extracted from cells, tissues, or other biological samples. Combined with Mass spectrometry, it is an efficient tool in proteome analysis. In this research, a proteome method to isolation and identification the proteins involved in female reproduction was estabolished and developed. Protein extractions from mouse ovary were run in IPGphor isoelectric focus system withllcm and 24cm long IPG strips respectively (pH3-10 L, 0.3mm thick). And then the proteins in ployacrylamide gels were to identify by Mass Spectrometry. This rapid high resolution and efficient technique to analysis should facilitate furthure investigation of biochemical mechanism of female reproduction.