The Study On Interleukin In The Correlated Tissue During The Replacement Of Primary And Permanent Teeth

The replacement of deciduous teeth and permanent teeth is an important phase during the development of teeth and maxilloface. There are two important physical phenomena in this process: one is the absorption of deciduous teeth roots, the other is occlusal movement of permanent teeth germs. Osteoclasts and odontoclasts play important roles in this process. Osteoclasts and odontoclasts are the only fuctional cells which can result in the absorption of deciduous teeth and alveolar bone. Their collective roles are to form the way of outgrowth and ensure the eruption of permanent teeth germs successfully. Previous studies showed that these two types of cells are very similar in the origin, structure and function. IL-1,4,6,8 can affect the formation and differentiation of Osteoclasts. Therefore, some scholars presumed that these cytokines can also affect the formation and differentiation of odontoclasts, then influence the absorption of deciduous teeth roots further. Till now, there are still lack of proof's about this hypothesis. That is why we carry out this experiment. Local dogs were divided into three groups: roots-absorbed 1/2 deciduous teeth group, roots-absorbed 3/4 deciduous teeth group, rootscompletely-developed permanent teeth adult dog group. Temporal and spatial expressions of IL-1,4,6,8 in pulp, periodontium of deciduous teeth and permanent teeth germs were observed using immunohistochemistry and in situ hybridization. These data were analyzed using hemi-quantity method. The roles of IL-1,4,6,8 protein and mRNA during the replacement of deciduous and permanent teeth were discussed. We hope to find new proofs which can illustrate the mechanism of replacement between deciduous and permanent teeth. Major contents and results are following:1. Expression and significance of IL-1 protein and mRNA during the replacement of deciduous and permanent teethDistributions of IL-1 protein and mRNA in pulp .periodontium and permanent germs in above 3 groups were observed using immunohistochemistry and in situ hybridization. The roles of IL-1 in the replacement of deciduous and permanent teeth were discussed.1. 1 There was no positive expression of IL-1 protein and mRNA in adult dog group. Positive expression of IL-1 protein and mRNA in roots-absorbed 1/2 deciduous teeth group and root-absorbed 3/4 group indicated that IL-1 participate in the replacement of deciduous and permanent teeth. The mechanism might be that it can promote the differentiation and maturation of osteoclasts and absorption of deciduous teeth roots and alveolar bone.1. 2 Strong positive expression of IL-1 protein and mRNA was found in roots-absorbed 1/2 deciduous teeth group. There was significant difference between roots-absorbed 1/2 deciduous teeth group androot-absorbed 3/4 group by image analysis system. This suggested that local concentration of IL-1 was high and facilitated the absorption of deciduous teeth roots and alveolar bone.1. 3 IL-1 protein and mRNA expressed positively in osteoclasts (odontoclasts), odontoblasts of deciduous teeth and periodontium. This indicated that these cells can translate IL-1 mRNA and synthesize the protein. Expression of IL-1 in odontoblasts has not been reported before.2. Expression and significance of IL-6 protein and mRNA during the replacement of deciduous and permanent teethExpression of IL-6 protein and mRNA in in pulp .periodontium and permanent germs in above 3 groups were observed using immunohistochemistry and in situ hybridization. The reles of IL-6 in the replacement of deciduous and permanent teeth were discussed.2. 1 There is no positive expression of IL-6 protein and mRNA in adult dog group. Strong positive expression of IL-6 protein and mRNA was found in roots-absorbed 1/2 deciduous teeth group. Weak or even negative expression of IL-6 was found in roots-absorbed 3/4 deciduous teeth group. This suggested that IL-6 participate in the remold of alveolar bone and promote the absorption of deciduous teeth roots.2.2 IL-6 protein and mRNA expressed po...