Expression Of EphB2 & EphrinB1 In Meningiomas As Well As Their Relationship To Angiogenesis And Peritumoral Brain Edema In Meningiomas

Objective To detect the expression of receptor tyrosine kinase EphB2 and its ligand EphrinB1 as well as their relationship to angiogenesis and peritumoral brain edema in meningiomas, and explore the possible mechanism of angiogenesis and peritumoral brain edema (PTBE) in meningiomas, and probe into a new strategy for antitumoral and anti-PTBE remedy. Methods Meningiomas tissue samples from 44 patients who underwent surgery at Daping Hospital were examined respectively for the extent of expression of immunoreactive EphB2&EphrinB1. In order to compare and statistically infer with meningiomas tissue samples, as control group, 14 normal meninges coming from patients experienced operation were also examined for immunoreation. Additionally, peritumoral edema was accessed on a subjective 5-point scale and blind basis radiographically from preoperative magnetic resonance imaging scans. Results1. The expression of EphB2 in the majority of 44 cases meningiomas as follows, 4 scale 7 cases, 3.5 scale 5 cases, 3 scale 6 cases, 2.5 scale 2 cases, 2 scale 8 cases, 1 scale 13 cases, 0 scale 3 cases, mean scale 2.19. The expression of EphrinB1 in the same meningiomas as follows, 3.5 scale 4 cases, 3 scale 3 cases, 2.5 scale 4 cases, 2 scale 15 cases, 1.5 scale 2 cases, 1 scale 11 cases, 0 scale 5 cases, mean scale 1.7. Ten of the 14 normal meninges have no expression of EphB2, only 4 show minimal (1 scale) expression of EphB2. Nine of the same 14 normal meniges have no expression of EphB1, only 5 of the same 14 normal meniges show minmal (1 scale) expression of EphrinB1.2. Angiogenesis in the 44 cases : 4 scale 2 cases, 3.5 scale 3 cases, 3 scale10 cases, 2.5 scale 4 cases, 2 scale 8 cases, 1 scale 10 cases, 0 scale 7 cases, mean scale 1.88.3. Thirty-three of 44 cases show different level of magnetic resonance imaging-assessed PTBE as follows, 4 scale 4 cases, 3 scale 11 cases, 2.5 scale 4 cases, 2 scale 3 cases, 1.5 scale 3 cases, 1 scale 8 cases, 0 scale 11 cases, mean scale 1.82. 4. There is positive correlation between EphB2 & EphrinB1 expressed in the meningioma cytoplasm and meningioma angiogenesis as well as peritumoral edeme.5. There is no correlation (p>0.05) between gender, age of patients & location of tumors and PTBE, angiogenesis as well as EphB2 & EphrinB1 expressed in the meningiomas. Meningiomas subclassified as meningothelial, fibroces and mixed show influence on expression of EphB2 and EphrinB1 in the cytoplasm of meningiomas (p<0.05), but there is no correlation (p>0.05) between subtype of meningiomas and PTBE, angiogenesis. Additionally, there is positive correlaton (p<0.05) between the size of tumors and PTBE, but no correlation (p>0.05) between the size of tumors, angiogenesis and EphB2&EphrinB1 expressed in the meningiomas. Conclusions 1. The majority of meningiomas expressed EphB2 and EphrinB1 in the cytoplasm.2. There is positive correlation between EphB2 & EphrinB1 expressed in the cytoplasm and meningioma vascularity, and it shows that EphB2 & EphrinB1 take part in meningioma angiogenesis.3. There is positive correlation between EphB2 & EphrinB1 expressed in the meningiomas and PTBE, and it suggests that EphB2 & EphrinB1 have part in peritumoral edema of meningiomas.