| PrefaceEssential hypertension ( EHT) is a common cardiovascalar disease. It's a severe public health problem. EHT can easily cause the complication of the heart, the brain, the kidney and so on, but the precise mechanism is not elucidated at present. Chlamydia pneumonia (C. pneumonia) is also called TWAR which was first described in 1986. C. pneumonia, the third species described in the genus chamydia, is an obligate intracellular pathogen. It is a common human pathogen which can cause respiratory tract infection. Several studies have demonstrated an association between C. pneumonia infection and cardiovascular disease and cerebrovascular disease, but the results are argued. To clarify the precise relationship between previous C. pneumonia infection and EHT, we investigated the epidemiological markers concerned with EHT and measured serum immunoglobulin G (IgG) antibody to C. pneumonia. Up to now, there hasnt been any report about the association between C. pneumonia infection and EHT in our country.Subjects and Methods1. Subjects: 62 patients with EHT were selected and at the same time 58 healthy controls were randomly selected in Beipiao city, Lia-oning province. All the subjects who had acute upper and lower respiratory tract infection, acute bronchitis or other lung infections were excluded from the study.2. The diagnosis standard of EHT: According to 1999 WHO -ISH guidelines for the management of hypertension, hypertension was defined as a systolic blood pressure (SBP) ^140mmHg and/or a dias-tolic blood pressure ( DBF) 5= 90mmHg. The measurement of blood pressure should be carried out according to the unified standard under standard conditions.3. The contents of epidemiological survey: We filled in the questionnaires by means of inquiring and measurement in sites. The contents of survey included: general characteristics, family history of hypertension, alcohol intake, smoking habits and so on. Five milliliters blood samples were drawn from an antecubital vein after an overnight fast. After centrifugation, the serum fraction was removed and frozen in aliquots at -70C until assayed.4. Detecting indicators and measuring methods;4.1 The presence of specific - IgG antibody to C. pneumonia was assayed using the solid - phase enzyme immunoassay ( EIA) kit commercially available from Labsystems company ( Labsystems, Helsinki, Finland). All the procedures must be strict with the instructions of the kit. The results are expressed as enzyme immunounit ( EIU). Used the formula for calculations: EIU8ample = [ ( A8ample-Ablank)/(Acal-Ablank ) ] x 130. The determination of IgG antibody to C. pneumonia; EIU <45 negative, EIU >45 positive.4. 2 The concentrations of serum total cholesterol ( CHOL) , high density lipoprotein cholesterol (HDL) , triglycerides (TG) were assayed by the kit commercially available from zhongsheng bio - tech-nical company (zhongsheng, Beijing, China).5. Statistical methods; Statistical analysis of dichotomous variables was by the chi - square test or Fisher s exact test. Student s t -test was used to assess differences between groups for two continuous variables. Multiple Logistic regression was used when evaluating the effect of a continuous or categorical covariates on a dichotomous outcome. A 2 - sided value of P <0.05 was regarded as statistically significant. The software used was SPSS10.0.Results1. Demographic characteristics in EHT group and control group General characteristics in two groups did not differ with respect toage, gender, body mass index (BMI) , smoking history, alcohol history , or serum HDL, TG. The family history of hypertension of EHT was more frequent than that of control group and the serum CHOL of EHT was also significantly higher than that of control group.2. Analysis of the positive rate of IgG antibody to C. pneumonia The positive rate of IgG antibody to C. pneumonia are 58. 1%(36/62) in the hypertension group and 75. 9% (44/58) in the healthy control group. The subjects with hypertension have the signif...
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