| ObjectiveThe purpose of this study was to explore the relation of serum fasting true insulin level and lipids in subjects with type 2 diabetes. The prevalence rate of diabetes mellitus is rising year by year, and cardiovascular complications is a main cause of morbidity and mortality. Dyslipidemia is more common in type 2 diabetes than that in the general population. The UKPDS research proved that the cardiovascular risk of type 2 diabetes could be partly decreased by strictly controlled blood glucose and blood pressure, but still had 59% of the diabetes patients who died of cardiovascular complications. Many persons think dyslipidemia is the most important and dangerous factor.The dyslipidemia in type 2 diabetes is difficultly controlled even if the blood glucose is normal and the dyslipidemia usually exists with hyperinsulinemia at the same time. Why it is is unknown. Some scholars think that hypertriglyceridemia is an earlier period of insulin resistance and insulin resistance is a center to cause dyslipidemia, furthermore , the happening of insulin resistance is often earlier than that of dyslipidemia.In the past, we usually use immunoreactive insulin (IRI) to e-valuate the relation of serum insulin, insulin resistance and dyslipi-demia and so on. However, the specificity of IRI is not so good because it includes proinsulin, and the cross - reaction is almost up to 30% ~ 100% , particularly in the patients of diabetes or impaired glucose tolerance. So IRI measurement value would be far higher than true insulin (TI) value and that misled the clinical worker to overestimate the insulin concentration in type 2 diabetes. Moreover, the sensitivity of IRIRIA can only attain 2-8 mU/L because it is affected by limited antibody competition principle. Therefore, its reliability makes person querying that using the IRI to calculate the parameters of insulin resistance index, insulin sensitivity, islet p - cell function index etc and to evaluate the relation of insulin level and lipids.With the development of monoclonal antibody and immunoanalys-is technique in recent years, there had been a new type of immuno-analysis method to measure true insulin in abroad. But at present the article that report the relation of insulin level, insulin resistance and lipids, blood pressure using true insulin at domestic and international is still very rare. This study adopt enzyme linked immunosorbent assay (ELISA) method that is specific and have no interference of proinsulin to measure the true insulin level in order to reflect the relation of each related factors more accurately. So it may provide a new thinking way for the diagnosis of cause of disease, prevention and treatment of dyslipidemia, diabetes and hypertension etc.Subjects and MethodsSubjectsCase group: we selected 113 patients with type 2 diabetes melli-tus who have no failure of islet B - cell function, no coronary heartdisease, no cerebrovascular disease, no cancers and no smoke. According to blood pressure level, we divide the case group into two groups; Type 2 diabetes group (T2DM, 67 patients) and type 2 diabetes with essential hypertension group (T2DM +EH, 46 patients).Control group; we also selected 30 normal persons who do not smoke as a control group.MethodsSpecimen collectionsAll subjects had not undergone any antidyslipidemia treatment in a month and antihypertensive treatment in two weeks. After at least 12 hours of fasting, blood samples were collected. Blood was drawn by venipuncture. Serum was separated by centrifugation at 3000 rpm for 15 minutes and was immediately frozen and stored at -20t until assayed for TI, IRI, lipids.Examination methodSerum TI adopted ELISA method, IRI adopted radioimmunoassay (RIA) , blood lipids and blood glucose adopted enzyme method. The results are expressed in mU/1, jxU/ml, mmol/1.Statistical analysisAll data were analysed by Excel 97, Foxpro6. 0 and SAS6. 12 statistical software. Enumeration data were analysed by x2 - test. All measurement data are expressed as the...
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