| Objective:To investigate the protection of anisodamine during intestinal ischemia reperfusion(I/R) and to examine the mechanism by which the multiple organ was damaged. Methods: Intestinal ischemia reperfusion was induced by clamping superior mesenteric artery for 120min period and then releasing arterial clamping for 2 hours or 6 hours. Anisodamine was administered intravenously 5 minutes before ischemic,60 minutes after ischemic and 5 minutes before reperfusion. Results: After intestinal ischemia reperfusion injury, the biochemistry increased which indicated that function of liver and kidney was damaged. The level of MDA was gradually increased, while the activities of SOD were gradually decreased in liver,lung and kidney homogenate. The level of MPO was gradually increased in in liver and lung homogenate. The serum NO,TNF-α concentrations after reperfusion were also significantly higher than those of sham-operated control group. A close correlation was shown between MDA content and NO content in serum. Furthermore, the results showed that pretreatment with anisodamine notably improved the function of organs, significantly lowed the level of MDA and improved the activity of SOD; and the level of MPO was lower than those of saline control groups. In addition, pretreatment with anisodamine significantly decreased NO,TNF-α concentrations in serum. Conclusion: This study demonstrated that intestinalischemia reperfusion may result in multiple organ damages, the mechanism may be involved in release of reactive oxygen species and bacteria or endotoxin translocation which in turn activated macrophages and PMN that can release abnormal mediators, then lead to SIRS. Anisodamine can protect organs against intestinal I/R injury, which may be associated with ameliorating reactive oxygen species-mediated organ damage, decreasing the aggregation and activation of PMN, inhibiting SIRS and inhibiting the release of endogenous NO.
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