Development Changes Of Glu,nNOS And GFAP Immunoreactivities In The Epilepsic Rat Brain By Kindling

Purpose:To explore the role of GliK nNOS and GFAP in the epilepsy mechanism. Methods:By pentylenetetrazol kindling in chemical method, we built the chronic epilepsy model. The rats are divided into control group and experimental groups. And the experimental groups included I-Grade group, III-Grade group ^ V-Grade group and V-Grade stop 24h group.With the immnohistochemical method, the morphology and the numbers of Glu ^ nNOS and GFAP immunoreaction positive cells in the hippocampal formation and frontal cortex were oberseved. By using the image analyzing system, the average optical density(AOD) of the immunoreactive production in corresponding regions of all the groups were measured.Results:(1) In the epilepsic rats of pentylenetetrazol-kindling, the numbers of Glu immunoreaction positive cells and the average optical densities(AOD) of thelll-Grade group and V-Grade group were increased compared with control group in the hippocampal formation and frontal cortex;On the other hand, compared with III-Grade group, both the observations of the V-Grade group were decreased in corresponding regions.(2) In the epilepsic rats of pentylenetetrazol-kindling, the numbers of nNOS immunoreaction positive cells and the average optical densities(AOD)of the V-Grade group and V-Grade stop 24h group were increased compared with control group in the hippocampal formation.The corresponding changes in frontal cortex were not found. (3) In the epilepsic rats of pentylenetetrazol-kindling, the numbers of GFAP immunoreaction positive cells and the average optical densities(AOD) of thelll-Grade group^ V-Grade group and V-Grade stop 24h group were increased compared with control group in the hippocampal formation and frontal cortex.Conclusion:In the pentylenetetrazol-kindling model, the increases of the expression of the Glu> nNOS and GFAP immunoreactivity were all observed in the hippocampal formation and frontal cortex, which suggested these results had some relations with the onset and development of epilepsy.