| Interleukin-1 (IL-1 ) is an important cytokin and regulating factor of polypeptides, which takes part in message communication among different kinds of Leukocyte. It also has abroad spectrum of biological actions and functions of regulating various immunoactive cells. Leukaemia inhibitory factor (LIF)is a kind of highly glycosylated secretary protein .It has various biological functions of stimulating the differentiation of marrow leukaemia cell line, at the same time inhibits the normol embryo stem cells differentiation and matains the reproductive function . The previous researchers thought that IL-I , LIF mainly came from blood monocytes and macrophages ,but mordern studies indicate that IL-I , LIF are also released from macrophages , granulosa cells ,thecal ovarian cells ,ect. And have a close relationship with reproduction .Nevertheless, there has been few research on the relationships for IL-I ,sex hormone infollicuar fluid and the results of IVF -ET until today .There is no report about the relationships of LIF in follicuar fluid of the PCOS patients with sex hormone and result of IVF -ET in our country .The enzyme-linked immunosorbent assay(ELISA) is used to quantify IL-1 , LIF in follicuar fluid , and time-resolved fluoroimmunoassay is used to quantify estradiol and progesterone in follicular fluid.The purpose of our study was to investigate prospectively the relationships and rules of IL-I ,LIF , sex hormone in follicuar fluid and the results of IVF -ET , evaluate the action of IL-1 , LIF in insemination ,embryo development , implantation and assess their clinnical values . Patients: From August 2000 to June 200 1 , Patients undergoing treatment for IVF-ET were recruited from the reproductive center of The First Affiliated Hospital of Zheng Zhou University . Part I: Pregnancy group consisted of 23 infertile women who conceived after undergoing IVF -ET treatment. No pregnancy group consisted of 33 infertile women who failed to conceive after undergoing IVF -ET treatment. Part II: The study group consisted of 11 PCOS Patients,and the control group consisted of 14 infertile women. The follicular fluid was obstain on the day of oocyte retrieval.Methods: 1. The enzyme-linked immunosorbent assay(ELISA) is used to quantify IL-1 , LIF in follicuar fluid .2. The time-resolved fluoroimmunoassay is used toquantify estradiol and progesterone in follicular fluid.3 .The methods of I VF-ET reference to the routin ways in our center[1] Result1 The relationships of IL-I P , LIF, sex hormorne in follicle fluild and IVF-ET results in pregnancy group and non-pregnancy group1 . 1 The concentration of IL-1 3 in follicular fluid of pregnancy group is (33. 62 +12. 18) pg/ral, significantly higher than that in non-pregnancy group ( 25.74 + 15.00 ) pg/ml (p<0.05) .but there is no difference between the two group for the concentration of LIF in follicular fluid .1.2 The level of IL-1 P in follicular fluid is positively correlated with the grade I embryo rate ( r=0.352 P=0.008) ,and the fertilization rate (r-0.299 p=0.025) .1.3 The level of IL-1 & in follicular fluid is positively correlated with progesterone (r^O. 520 pO.OOl) .E2/P ratio is negatively con-elated with the fertilization rate(r=-0.364 p=0.006).1 .4 The level of LIF in follicular fluid is not correlated with the grade I embryo rate, the fertilization rate and Progesterone.2 The relationships of IL-I P , LIF, sex hormorne in follicle fluild and IVF-ET results in PCOS group and control group2.1 The concentration of LIF in follicular fluid of PCOS group(21.07pg/ml) is significantly lower than that in control group(33.53pg/ml) (p<0.05) . The concentration of IL-1 & in follicular fluid of PCOS group( 39.93pg/ml) is significantly higher than that in control group(28.30pg/ml) (p<0.05 ) .2.2 The concentration of Progesterone in follicular fluid of PCOS group(l 9 1.85X1 03nmol/L) is significantly lower than tha...
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