Study On Pharmacodynamics And Quality Standard Of Chuankang Injection

This paper studied the plant resource, quality evaluation, chemistry, pharmacology andect. al of Berberidaceae Epimedium L. The result indicate that it is a large distribution ofEpimedium, large amout of yield, and flavonolignan is the mainly effective chemical substance. The researcher of home and abroad had studied 13 varieties of this genus, got over 100 kinds of components, and had versatile pharmacologic effect. Pharmacologic experiment and clinical study indicate that Epimedium may increase blood stream of coronary vein, increase humoral immunity and cellular immunity, increase DNA synthesis rate, effect nucleic acid metabolism, antisenescence, may cure mouse' cough and gasping that caused by augmented and acetylcholine. The paper use the abundant resource of Epimedium, developed a new preparation, and studied the quality standard.The paper is the first one that invented a injection according to the calm-gasping effect of Epimedium, studied the extract process of preparation, developed an optimal process of extraction with traditional medicated process and modern technique, the method is water extraction and ethanol precipitation, water washing-out supernumerary substance in polyamide column, collect ethanol elution. It is obey the rule of Chinese Medical, extract the effective component and simplify the process at the same timeThe paper studied the effect and general pharmacology of Epimedium injection comprehensively and deeply. The effect research indicate the Chuankang injection had distinct effect on calm cough of mouse that caused by ammonium hydroxide; may calm down the gasping; also it can release the air tube smooth muscle distinctly. The injection had antianaphylaxis activity. Increase humoral immunity and cellar immunity action of chronic trachitis model animal distinctly, it also has some tense of anti-inflammatory action and bacteriostasis action. It can increase stress ability of sensitizer.Toxicology experiment result indicate that LDso of hypodemic injection mouse is difficult to detect, has fairly low of toxicity. Long-time toxicology experiment showed that the injection have no distinct effect on animals physiogmonic activity, blood, urine, blood serum, heart beating rate, blood pressure and kidney function, no distinct toxic and side effectThe paper studied the quality standard of Epimedium injection firstly, present the scientific method for develop Chinese Medicine injection. Quankang injection is a kind of Chinese Medical injection for cure chronic bronchitis and asthema, it composed of Epimedium et al. herbs. The active substance of Epimediumis distinctly, so the study of this quality standard mainly to test the flavonoid and some glucose of Epimedium. This paper use ultraviolet spectroscopy, HPLC and spectrophotometric method to determine the content of total flavonoid, glucose and icariin. Content determination of this injection is that total flavonoid 1.28mg/ml, icariin o.48mg/ml, total glucose 0.3mg/ml. Present the evidence to quality control of the preparation.On the other hand , according to the guide rules of Chinese medical injection in new medicine evaluation, to make sure this injection is safety, effective and stability, assurance the quality of the injection, and found the base of injection's fingerprint.