Study On The Expression Of Bcl-2,p16 And PCNA In Childhood Acute Leukemia

Objective: To study the expression of bcl-2 p16 PCNA in childhood acute leukemia and their relationship, and to explore the role of apoptosis and proliferation in the pathogenesis of acute leukemia and their clinical significance.Methods: The expression of bcl-2 p16 and PCNA in paraffin sections of bone marrow biopsy samples were studied by immunohistochemistry in 44 cases of children with acute leukemia (including 29 cases in initial diagnosis and 15 cases in complete remission) selected as test group and in 19 cases of children without leukemia served as control group.Results: (l)The positive expression rates of bcl-2 were 65.5% 26.79% 15.8% in primary diagnosis group complete remission (CR) group control group respectively. The-positive expression rate of bcl-2 in primary diagnosis group was significantly higher than that in control group and CR group, but there was no obvious difference between CR group and control group. (2) The positiveexpression rates of p16 were 37.9% 73.3% 89.5% in primary . diagnosis group CR group control group respectively. The positive expression rate of p16 in primary diagnosis group was obviously lower than that in control group and CR group, while there was no apparent difference between CR group and control group. (3) The positive expression rates of PCNA were 75.9% 40.0% 26.3% in primary diagnosis group CRgroup control group respectively. Thepositive expression rate of PCNA in primary diagnosis group was significantly higher than that in CR group and control group, but there was no distinct difference between CR group and control group. (4) Either the expression of bcl-2 or the expression of p16 was positive in most of acute leukemia patients, and so was the expression of p16 and the expression of PCNA, whereas the expression of bcl-2 and the expression of PCNA were together positive or negative in most of cases.Conclusions: Apoptosis inhibited by bcl-2 protein high expression, proliferation enhanced by p16 protein deletion, and proliferative activity of cells raised by PCNA overexpression might play a very important role in the leukemogenesis and progression of acute leukemia. There were some kinds of relationship among the expression of bcl-2 p16 and PCNA, and thechange of their expression level before and after treatment might help to judge the effect of treatment. The expression level of bcl-2 protein p16 protein and PCNA were associated with prognosis in newly diagnosed childhood acute leukemia patients.