Resveratrol Content In Berries Of Chinese Wild Vitis Species

Resveratrol content in 11 Chinese wild Vitis species encompassing 36 accessions grown in natural vineyard conditions was determined with high pressure liquid chromatography. Resveratrol content has also been analyzed in four cluster organs (berry skin, berry flesh, seed and pedicel), in berries of different developmental stages, and in healthy and Glomerella cigulata infected berries, with 3 accessions of V. quinquangularis and European cultivars.The main results from this study were as follows.1. Resveratrol concentration in berries existed differences among Chinese wild Vitis species and among accessions. Resveratrol concentration was the highest in V. quinquanglaris (0.358-7.518 u g g-1); Dan-2, 83-4-67 and Shang-24 accessions of V. quinquanglaris contained much higher resveratrol than the other accessions, their resveratrol concentrations were 7.518 U g gl, 7.208 U g g-1 and 7.173 U g g-1 respectively. Resveratrol concentration could not be detected in V. davidii var., V. thunbergii, V. yeshanensis, V. sinocinerea and V. sp. (Maihuangfuye) grape, and in Jinan-1, Fujian-4, Lueyang-4 and Xuefeng accessions of V. davidii, Hunan-1 and Guangxi-1 accessions of V. pseudoreticulata, and Meixian-6 accession of V. piasezkii.2. With Dan-2 accession of V. quinquanglaris and Pinot Noir as studied materials, resveratrol concentration in berries showed differences in different developmental stages. Resveratrol concentration was the highest when berries were ripe; and it increased when berries came into veraision.3. Resveratrol concentration was significantly different in four cluster organs. Resveratrol concentration was the highest in pedicel (10.667-37.288 u g g-1); and it was higher in berry skin (4.6718.543 U g g-1) than in seed (0.584-0.761 U g g-1) and in berry flesh (0.208-0.574 U g g-1).4. Berries slightly infected by Glomerella cigulata contained higher resveratrol than the healthy ones. Glomerella cigulata infection may induce resveratrol production in grapes.5. Climate had some important effects on resveratrol content in grapes. However, the genotype of grape species and variety may be one of the mos t important factors in resveratol production in grapes.6. A simple and reliable reversed-phase HPLC method was improved, and it was used to determine resveratrol content in berries and wines in this research.