Study On The Anti-tumor Effect In Vivo Experiment Of GPS And Its Molecular Mechanism

Objectives: Polysaccharide is the high polymer compound, which is made up with hundreds of monosaccharide by glycosidic linkages. It is one of the most important things to life. Glycyrrhizia polysaccharide (GPS) is a kind of plant polysaccharide that was discovered by Chinese scholar in recent years. It can active the immuno-system of the organism, resist virus, and extend lifetime of the mouse which was injected with EAC. In order to understand the bio-activity of GPS and discuss the function of the polysaccharide which gets out from Chinese herb in the anti-tumor therapy, we collect tumor specimen from animal experiment, use immuno-histochemistry and FCM to study the difference among mouse whether be given GPS or not.Method:1. Animal experiment: 40 pure breed Balb/c mouse which were injected tumor cell, randomly divided into 4 groups. Group A is given Saline, group B is given CTX, group C is given GPS, groupD is given GPS and CTX.2. Collect the tumor, calculate the anti-tumor rate. Measure and caculate the volume of the tumor, draw the tumor growth graph.3. Collect the spleens, calculate the weight4. Use FCM to check apoptosis of the tumor cells of the mouse5. Use immuno-histochemistry method to check the expression of Bax> Bcl-2 and VEGF gene in the tumor tissue.6. Statistics method: use SPSS software to calculate the data, significance level chosen was p value less than 0.05 (p<0.05), and all were two-sided.Results:1. The tumor weight of group B >. C and D are lighter than group A. It differed significantly between them. The graph indicated that the volume of group A was bigger than that of group C and D.2. The spleens' weight between these groups has no difference.3. In the tumor cells' graph of group C and D, we can see apoptosis peak, but we can't see it in graph of group A4. The frequency of Bax> Bcl-2 and VEGF gene expression differedsignificantly among those groups.Conclusion: As a kind of herb polysaccharidc, GPS can resist tumor with chemical-therapy drug, induce the tumor cells to apoptosis. It can be developed to be a new anti-cancer drug.