Regulatory Effects Of Electroacupuncture On Gastrointestinal Mucosal Endocrine-immune Network

Acupuncture can regulate the visceeral fiinction.The stomach channel of foot-Yangming(St),part of the stomach and coordinating the viscera,can be used for the treatment of gastrointestinal diseases.Lots of clinical and experimeatal studies have proved that stimulating the acupuncture points of St produces good regulatory effect on gastrointestinal function,and cures gastrointestinal injuries and after conditions.However.its true mechanism still remains unclear.With feather investigation and studies,more and more attention is paid of the gastrointestinal mucosal immunity.But the research into acupuncture's effect on gastrointestal mucosal immunity is reported less.So in this sudy,based on synchronous experimeat,we stimulate the acupuncture points of St and measure its corresponding biochemical levels of gastrointestal immune system in order to evaluate acupuncture's effect on the gastrointestal mucosal immune system and its possible mechanism. Methods40 rats were randomly divided into 4 groups :Tsusanli group.Shangchusu group,non-acupoint group and non-acupuncture group.Then the changes of brain-gut peptide and gastrointestinal mucosal immunity were synchronously observed.Radiommunoassy was used to determine the levels of SP,VIP,SS,sIgA in peripheral blood and gastrointestal tissue.The immunohistochemistry staining to observing SP,VIP,SS expression in the gastrointestal tissue.The proliferatio activity of IEL and LPL were detected by mononuclear cell cytotoxicity assay. Results1, EA on acupoints of St increased levels of slgA in peripheral blood and gastrointestal tissue,and so gastrointestal immunological function wasimproved,especially when Tsusanli point stimulated. 2> EA on Tsusanli increased lEL's and LPL's prolifeation activates.Tongether with slgA.IEL and LPL could reinforce the gastrointestalimmunological function. 3, EA on acupoints of St increased the levels of SP.VIP.SS in peripheralblood and gastrointestal tissueAccording to our experinental resultsand related literaturre,brain-gut peptide after acupuncturre can regulategastriintestal immunologic function through the peripheral roate andgastrointestal local effect. ConclusionsEA on the points of St produced good regulatory effects and the effects aried with different poines,which meant some points had relative visceral speaificity;it was proved that EA on Tsusanli enhanced significantly gastrointestinal immunological function, and brain-gut peptide and endocrine system wre also involved in the regulatory effect,the above factors interact each other and regulate effectively the gastrointestinal endocrine immunological network.