The Role Of Mycoplasma Pneumoniae In The Exacerbated Stages Of Asthma, Chronic Bronchitis And Community Acquired Pneumonia Patients And Comparing Serum Concentrations Of IFN-γ,IL-6,TNF-α In These Patients

Objective: To explore the relationship between pulmonary Mycoplasma Pneumoniae(MP) infection and acute exacerbated stages of Asthma,Chronic Bronchitis(CB),Community Acquired Pneumonia(CAP), compare different serum concentrations of IFN- , IL-6 TNF- a in these patients and show if MP infection works on the cytokines of the patients.MethodsiSerologic analysis through enzyme-linked immunolosorbent assay(ELISA) were used to detect MP-IgM and MP-IgG to determine the presence of MP and the serum concentrations of IFN- Y x IL-6x TNF- a in 30 healthy control subjects, 34 patients with acute exacerbated stage of Asthma, 31 patients with acute exacerbated stage of CB and 34 patients with CAP. Sputum MP polymerase chain reaction(PCR) was also used to determine the presence of MP. The positive rate of MP infection and the serum concentrations of those cytokines were compared between the different group patients.And the difference of the cytokines was also compared between MP positive and negative patients in the same group patients. Results:!.The positive rates of MP by ELISA to detect MP-IgM were 35.3%, 29.0%, &.&%, 0% in the patients with CB, Asthma, Pneumonia, healthy controls respectively; by PCR were 29.4%, 25.%%, %%%, 0% and by combining methods with MP-IgM,MP-IgG, PCR were 5K.K%, 58.1%, 26.5%, 0% respectively.The positive rate of CB or Asthma patients was much higher than that of CAP(P<0.05) and healthy control subjects while there was no difference between the asthmatics and the chronic bronchitics(P>0.05) according to MP-IgM or combing methods mentioned above.There was no difference(P>0.05) about positive rates between thethree group patients according to MP-IgG regarded as preceding infection index. 2. The serum concentrations of IFN- y ^ IL-6 and TNF- a in the patients with CB, Asthma, Pneumonia were much higher than in the controls(P<0.05). 3. The IFN-Y concentrations of pneumoniae patients increased much higher than those of Asthmatic and Chronic Bronchitic ones while there was no difference between the latter two groups.MP infection did not greatly affect the serum IFN-Y level of the three group patients (P>0.05) 4.The CB patients and Pneumoniae ones had significant higher level of IL-6 than the Asthmatics had(P<0.05). There did not show much correlation between MP infection and the IL-6 level of patients with CB and Pneumonia(P>0.05) but the asthmatic ones did and ascended its level (P<0.05).5. The CB patients had higher serum concentrations of TNF-a than the Pneumoniae ones had(P<0.05). MP infection made no significant affections on the serum level of TNF-a in the CB and Asthmatic patients(P>0. 05) while on the contrary it did in the Pneumoniae ones. And MP infection decreased the TNF-a concentration of the patients(P<0.05).Conclusions: l.MP was present in the subjects of acute exacerbated stages of Asthma and Chronic Bronchitis with greater frequency than Community Acquired Pneumoniaic and control subjects, and may play an important role in the pathogenesis of the former two diseases.2. There existed celluar immune function disorders with the decreasing of protective factor IFN- Y and great promotion of severe inflammatory factors TNF-a , IL-6 in the subjects of Asthma and Chronic Bronchitis. 3. MP infection made no significant affections on the cytokines such as TNF-a and IFN-Y in the subjects of Asthma and Chronic Bronchitis and it may act on their immune systems by other ways.