| Background: A shortage of lung donors pool is a serious obstacle to the widespread application of lung transplantation for end-stage lung disease. The desire of enlarging the donors pool compels us to explore the feasibility of non-heart-beating donor in rat lung transplantaion.Methods: Sixty Sprague-Dawley rats were randomly divided into 3 groups that lung of the rat were harvested immediately, 30 minutes and 60 minutes after cardiac-arrest. All the donor heparinized lungs were ventilated at the moment when they were flushed with the low potassium dextran solution and then stored in the same solution at 4 for 4 hours. The recipient animal underwent left thoracotomy, and then orthotopic left lung transplatation. The right pulmonary hilum were ligated 1 hour after reperfusion.Results: All the recipients of heart-beating lungs and 30 minutes cadaver lungs survived the observation period with excellent gas exchange, whereas 4 of 10 recipients of 60 minutes cadaver lungs survived for 10 minutes after the ligation of right pulmonary hilum and 3 for 20 minutes. The microstructure, pulmonary compliance, energy metabolism and other markers show no significant difference between HBD group and NHBD-30 group.Conclusion: The adoption non-heart-beating donor can be a safe and effective method to increase the lung donor pool. Since the ischemia-reperfusion injury shows more serious with the longer warm ischemic time, there should be a definition for the warm ischemia time, such as 30 minutes for the rat. |
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