| When through GI into S phase, the progression of the cell cycle is governed by a restriction checkpoint. Its function failure will cause cell that should stop grow up or generate physiological apoptosis in normal condition into the cell cycle, result in cell uncontrolled proliferation. P27, pi6 are the members of cyclin-dependant kinase inhibitors that negatively regulated cell through Gl into S phase, inhibited cell proliferation. Cyclin D^ cyclin E inversely activate progression of the cell cycle, accelerate passage of Gl into S phase. The disturbance of the cell cycle has relationship with neoplasms such as breast cancer, pulmonary carcinoma, colonic cancer and so on, abnormal expression of cell cycle regulating proteins were confirmed by many researches, and these proteins change could be important in tumorigenesis, may be as a molecular marker of prognostic of these disease.Ki-67 scores as a cell proliferative marker can be well predict activity and quantity of proliferative cell. For overall evaluate regulating proteins of the cell cycle action in tongue tunnorigesis and clinicopathologic significance in tongue cancer, two group of proteins which have inverse action on the cell cycle were chosen as targets of this research: cyclin DI ? cyclin E and p!6x P27.Objectives of this research include: 1. Examined expression of cyclin Dj, cyclin E, p27, p!6, Ki-67 protein in normal mucosa, dysplasia, squamous cell carcinoma in tongue; 2. Investigating expression of cyclin DI, cyclin E, p27, p!6, Ki-67' s relationship with pathological type, metastasis, TNM in tongue squamous cell carcinomas; 3. Investigating expression of cyclin DI, cyclin E' s relationship with expression of p27, p!6 proteins in tongue squamous cell carcinomas; 4. Investigating Ki-67 scores' relationship with expression of cyclin DI, cyclin E, p27, p!6 protein in tongue squamous cell carcinomas.Materials and Methods1. 31 dysplastic epithelias and 58 squamous cell carcinomas in the tongue were selected. Information of these cases was collected including age, gender, location, pathological type, metastasis and TNM stage. 10 normal lingual mucosas were used as controls.2. Using immuohistochemical SP staining, we examined cyclin DI, cyclin E, p27, pi6 and Ki-67 protein expression in paraffin slices of 31 dysplastic epithelias and 58 squamous cell carcinomas in the tongue. As controls, expressions of these proteins in 10 normal lingual mucosas were also detected.3. Difference of cyclin DI, cyclin E, p27, p!6 and Ki-67 protein expression were analyzed in normal mucosas, dysplastic epithelias and squamous cell carcinomas of the tongue. Relationships of these protein expression with pathological type, metastasis and TNM stage were also analyzed in tongue cancer, and so were relationship of pi6, p27 expression with cyclin DI, cyclin E expression.Results1. cyclin DI positive expression in dysplasias and tongue cancers was 32.26% and 60.24% as negative expression in normal controls, there was a statistical difference between normal controls and dysplasia group (P =0.040), and between normal controls and cancer group (P=0.000), and between dysplasia and cancer group ( x 2=9.651, P=0.003); the positive cyclin DI rate in LN- and LN+ group was 45.95%, 85.71 %, in Gl and G2, G3 group was 44.44%, 86.36%, in (I + II) and (HI+IV) was 53.12%, 69.23% respectively, There was a statistical difference between LN- and LN+ group ( x 2=8.854, P=0.003), and between Gl and G2, G3 group ( x 2=10.027, P=0.002), and no statistical difference between (I +11) and (III+W)group; Ki-67 score in cyclin DI negative and positive group was 33.496+9.875 > 56.327?16.524 (t=5.884, P=0.000).2. Cyclin E was negative expression in normal controls or dysplasia group and positive rate was 43.10% in tongue cancer, the Cyclin E positive rate in Gl and G2, G3 group was30.56%, 63.64%. There was a statistical difference Gl and G2, G3 group (x 2=6.093, P=0.014), and between (I + II) and (ffi+IV) group ( x2=4.090, P=0.043) , and no statistical differencebe... |
