| Injured cataract is one of the most important complications, with the morbidity of 36%-52%, induced by the perforating or concussive wounds in eyes, which is also the leading cause of the blinding, about 28.2% of all the visual disturbances. At present, the common viewpoint considers the lens epithelial cells' (LECs) proliferation, migration and differentiation caused by the mechanical trauma as the important pathogenic factor. And people pay more and more attention to the activities of the extra cellular matrix (ECMs) recently, due to the newborn of the conception of extra cellular stroma Matrix metalloproteinase (MMPs)/ tissue inhibitor of metalloproteinase (TIMPs) system plays the key point of many physiological and/or pathogenic procedures as the main enzyme system to degrade the ECMs. As a kind of secreting multifunction factors, the TIMPs not only specifically restrain the activity of the MMPs, including collegenase, gelatinase A, B, and stomelysms, but inhibit the activation of the MMPs. Due to their characteristics mentioned above, TIMPs play critical roles in various physical and pathogenic process such as inhibiting thevascuiarization, blocking the cellular migration and anti-inflammation, fn addition. recent experiments suggested that the TIMPs also have the direct growth factor like effects.ft is the hot spot to study the roles of the MMPs/TIMPs system in oculopathy in recent years, and many experiments have confirmed the abnormal expression of the TIMPs in various optical diseases. But data of systematic study is absent on the changes of the expression of the TIMPs after the perforating injure in eyes and the possible role of them in the occurrence of the injured cataract. The purpose of this study is to establish the animal model (rabbits) of perforating cataract, then to detect the dynamic changes of the TIMP-1,2 in the optical tissues , such as the aqueous humor, iris and lens capsules, thereby to discus the mechanism of the TIMPs in the cause of injured cataracts.1. Animal model of injured cataract:The model was established by puncture the rabbit's eye ball directly into the lens by sterilized needles , which resulted in opacity of all the wounded eyes and, in some eyes, the accumulation of the LECs and ECMs under the posterior capsules, which suggested the perforating injures were the certain cause of the complicated cataract.2. the expression of the TIMP-1,2 in the ocular tissues:All the rabbits were randomly divide into four groups, and killed in the first, third, seventh and fifteenth days after being injured by injection of air to get the tissues (aqueous humor, iris and anterior capsules), the aqueous humor and iris were stored in -80 癈, while the anterior capsules were used to make the stretched preparation of lens epithelial cells.2.1. The expressions of TIMP-1,2 in the aqueous humor and iris:We detected the TIMP-1,2 in the aqueous humor and iris by the method of reverse zymagraphy, and found the proteins' content mushroom within 24 hours and disappeared in the third day in both tissues. Scan the gels and measure the optic densities of the straps of 21.7KD and 28KD, which suggested the expressions of the proteins have significant differences between the injured group and the control group (p<0.01)2.2. The expressions of the TIMP-1,2 in the lens epithelial cells:Separate the anterior capsules with attached LECs into two parts to make the stretched preparation of lens epithelial cells, and immunohistochemical analysis of TIMP-1,2 were processed with S-P technique, the positive area integral optic density (IOD) were measured by the Mias multifunctional quantitative analysis softwareThe TIMP-1,2 were founded in the cytoplasm and extra cellular matrix in injured groups in first, third and seventh day separately , mainly in the cytoplasm While in the control group , no positive particle were founded The quantitative analysis results showed there were significant differences between the injured and control groups (p<0 01) in the first, third and in the...
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