| Immoderation drinking of long-term is the most important reason of human alcoholic liver injury, surfeit can break the liver cell, and the different processes of pathological changes------alcoholic fatty liver, alcoholic hepatitis, alcoholic hepatic fibrosis and alcoholic hepaticcirrhosis. Although up to now, no enough effective therapy means have been found to cure alcoholic liver disease(ALD), but with the new pathogenesis research progression, treatment means continuously obtain new progress. The tea polyphenol(TP) is separated from the inside of natural plant tea, purificated from the compound of the polyphenol complex. In all countries of the world, such as China, Japan...etc.,many research experiment to the polyphenol have been carried out. The research discover that it has various significant pharmacological function, particularly in protecting liver, anti-oxidize and regulating immunity. According to pharmacological function character of the tea polyphenol, This animal experiment research is to observe its aegis function to alcohol liver hurt animal model, and to study relationship between the pharmacological function of the tea polyphenol and the alcoholic liver disease.Material and methods:Feeding with the standard of cleanliness class II in the Zhejiang University medical college animal center,120 S-D rats, weighting 180-210g, were divided into five groups randomly( A, B, C, D and E group),A group was took as alcohol group; B, C, D groups was took as TP group and E group was took as control group. Each rat in group A, B, C and D was given 56% alcohol with the dosage of 7g/ kg respectively everyday, meanwhile, B, C, D groups rats were given TP with the dosage of 0.05 .. 0.125^ 0.25g/kg everyday morning. The E group was only given with same deal of drinking water everyday. The mental state, movable circumstances, hair condition, taking meal and excreting of each rats were observed in experiment.In the 4,12 and 24 weekend, rats were killed in batch, and rats vein blood wered collected. The rat liver specimen was fixed in 10%formaldehyde solution. Then took the serum specimen to evaluate:!. Liver function: Alanine aminotransferase(ALT) and Aspartate aminotransferase(AST);. 2. antioxidant quality measurement: superoxide dismutase(SOD), malondialdehyde(MDA)and glutathin(GSH). 3. determing the serum endotoxin level. The liver specimen were organized to be fixed, embed , sliced and the HE stain, Masson three color stain. Then all speciman were put to observing the hidtology change, including steatosis, alcoholic hepatic inflammation (degeneration, necrosis of liver cells and Mallory body ) and collagen deposition梙epatic fibrosis degree. Semi-quantitative and quantitative scoring methods were used to analyze those histological change.Results:There were totally 87 rats survival in the experiment.( all dead rat were in alcohol group, all control group rats were surviving.) At the beginning of experiment, after given alcohol, rats showed drooling, drowsiness, anorexia, depressed, decreased food appetite and retardation. All this phenomenon would extinction in 4-5 hours. With the experiment going on, politure of rats hair were getting worth, the weight increasing slowly, most of themdiarrhoeaed. But in TP group, after drinking, rats awoke early than alcohol group. After 2 months, all above phenomenon were lessen than before. Rats in control group were in good mental state, good appetite, without drowsiness phenomenon, and with normal bowels.The AST level in 4th, 12th and 24th week alcohol groups were higher than control group. AST in 4th week low dosage TP group were much higher than control group, but in median and high dosage TP group ,its level were obviously lower than and pure alcohol group. In 12th weeks low, median and high dosage TP group , the AST level is much lower than pure alcohol group. In 24th weeks low and median dosage TP group, AST level were higher than control group, but in high dosage TP group , the AST level is significant lower than low .median dosage TP group...
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