| Uterine cervical carcinoma is the most common genital malignancy. The etiological factor is not completely clear. A strong association with human papillomaviruses (HPVs) has been suggested. The genes including p53, p!6 and pRb are anti-oncogenes, their proteins play important roles in Gl/S check point, and can block cell period in Gl phage .The genes mutation and abnormal expression about p53, p!6 and pRb have been found in human malignancy. The MDM2 gene is an oncogene, whose protein can inactivate the wild type p53 protein in vivo. The MDM2 gene amplification and over-expression have been found in human malignancy.54 normal cervix have been selected as the control group while 155 specimens as the experiment group including 47 cervical intra-epithelial neoplasia (CIN), 61 invasive squamous cervical carcinoma of the uterine cervix (SCC), 35 adenocarcinoma of the uterine cervix(AUC), 12 adenosquamous carcinoma(ASC) of the uterine cervix. To study their role and correlation in uterine cervical carcinoma, HPV6/11 and HPV 16/18 were investigated by in situ hybridization(ISH), and p53, MDM2, p!6, pRb proteins were investigated by immunohistochemistry in the serial section of specimens.Part one. HPV infection and cervical carcinomaMethods: HPV6/11 and HPV16/18 were investigated by in situ hybridization (ISH) in 209 specimens above-mentioned. Statistical analysis includes X" test and Fisher's exact test.2002 ^ismResults: The positive rate of HPV6/11 was 20.37 % (11/54; in the control group, and in thefour experiment group including CIN 46.81% (22/47,) , SCC 42.62% (26/6\) , AUC 34.29%(12/35) ,ASC 66.67% (8/12; , there was no significant difference (P>0.05; , but there wassignificant difference between the control group and the experiment group (PO.05; except AUC.The positive rate of HPV16/18 was 16.67% (9/54; in the control group, and in the four experiment group including CIN 70.21% (33/47;, SCC 62.30% (38/61;, AUC 34.29% (12/35;, ASC 66.67% (8/12; . There was significant difference between the control group and the experiment group including CIN, SCC and ASC (P<0.05; except AUC. Significant difference was also be found between SCC and AUC (PO.05; , but not be found between CIN and SCC (P>0.05; . The positive rate of HPV16/18 was higher than HPV6/11 in CIN and SCC, but not in the control group, AUC and ASC.Conclusion: (1) HPVI6/18 infection has a strong association with SCC and CIN; (2) The relationship between HPV16/18 infection and SCC was more close than AUC; (3) ASC is possible related to HPVI6/18 infection .Part two. p53, MDM2 protein expression in cervical carcinomaMethods: Expression of p53, MDM2 protein was studied by immunohistochemistry in 54 cases of the control and 155 cases of the experiment group. Statistical analysis includes X test, Fisher's exact test, Mann-Whitney test and spearman's rho test.Results: The p53 positive rate was 3.70%(2/54) in the control group ,and 19.15 % (9/47; in CIN, 26.23% (16/6U in SCC, 20.00% (7/35;inAUC, 16.67% (2/12;in ASC. Thep53 positive expression index between the experiment group and the control group was significant (P<0.05), while the difference in the four experiment group was not significant ( P>0.05). The p53 positive expression index was not related to CIN grading (P>0.05 ), and has no significant difference in histological differentiation of SCC and AUC (P>0.05). The case of p53 positive rate >25% was respectively 14.29% (5/35) in AUC, 4.92% (3/61) in SCC, and 0 (0/12) in ASC.The MDM2 positive rate was 7.41 %(4/54) in the control group , and 14.89% (7/47; in CIN,200226.23% (16/61) in SCC, 11.43% (4/35) inAUC, 0 (0/12) in ASC. There was no statistical difference between the control and CIN, AUC, ASC (respectively P> 0.05), but in SCC (PO.01) positive rate is higher than in the control, there was no statistical difference between groups of the experiment (P>0.05) except SCC and ASC (P<0.05). The MDM2 positive expression index is not related to CIN grading (PX).05), and has no significant differe... |
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