| Peripheral nerve injury is commonly encountered clinically in wartime and peacetime, causing extensive disability and posing a complex challenge to surgeons. Since 1960s with the development microsurgery technology and it's wide application in the repair of peripheral nerve injury, the outcomes of patients following surgical repair of injured nerves were improved yet still not satisfying.In the 1980s with the rapid development in neurobiology, research of peripheral nerve injury was deepened into cellular and molecular levels. Currently there is a number of research in the literature detailing in neurotropism. neurotrophism, microenvironment of injured peripheral nerve and reports about the effects of neurorrophic factors, growth factors and schwann cell transplantation in promoting nerve repair. All this indicates the focus of peripheral nerve injury research is transferred from anatomic repair of injured nerve to a new potential means as microenvironment treatment.Natural cell growth control factor (NCGCF) is a new rapid growth factor that is extracted from animal tissues by advanced biochemistry technology. A number of large case animal experiment, biochemistry detecting, clinical practice have proved that it can expedite the repair process of damaged tissue by promoting and intensifying microvascular regeneration, stimulating the bioactivity of related cells.This is the very fist time that we are honored to have an experimental investigation of the effect of NCGCF on peripheral nerve injury repair. Our research is designed to determine the therapeutic effect of NCGCF on repair of injured peripheral nerve and discuss the underlying mechanismDissertation for master's degree, Zhejiang UniversityMaterials and methods1.Material and equipment(1) Material: 65 adult female SD rats weighted 200-23l)g provided by Experimental arumal center of Zhejiang University. School of medicine. Anaesthetics: ketamine hydrochlonde(2ml:100mg). Agent: NaCK CaCk NaHC03, MgS04:Glucose. Others: distilled water, saline, alcohol, swab, et al.(2) Equipment and Apparatus Conventional surgical device Walking tracts analysis apparatus (DIY) Standard Bath systemElectronic analysis balance2.Surgical process and animal modelAll adult female SD rats are weighted before anesthetized via intraperitoneal adminstration of Ketamine Hydrocholoride (40mg/kg body weight). The right gluteal region was shaved and swabbed with alcohol. Using a gluteal-splitting approach . the sciatic nerve was exposed and isolated, a special flat-jawed microvascular clap was used to crush a 3mm segment of the prepared nerve with a time of 20s. All nerves were kept moist with salinethroughout the procedure. Wound closed, animals are allowed to recover from the anaesthesia\ and placed in cage.3.Group and administration methodThe animals were randomly divided into3 groups.Group A(n=5)underwent a sham operation without crush or agent administration. Group B(n=30)each animal received injections of NCGCF 0.2ml in volume by subcutaneous injection 24h and Ih prior to nerve crush and then daily for the following 7 days. Group C(n=30)received injections of saline in volume equivalent to drug used in group B and followed the same protocol as group B.4.Evaluation of results(l)Motor functional assessment was performed on days l,4,7,10,14,21,28,35and 42 after nerve crush in all rats. Animals were evaluated by a walking track test: briefly the animalsDissertation for master's degree, Zhejiang Universitywere allowed to walk down a walking corridor leaving black footprints on the paper. The distance between the first and fifth toes (TS) ,the distance between the second and fourth toes (IT), and the print length(PL) were measured. The sciatic functional index (SFI) was calculated according to the following equationSFI=109.5* ( ETS-NTS) /NTS-38.3*(EPL-NPL)/NPL+13.3*(EIT-NIT)/NIT-8.8(2)Muscle contractility testing was performed in groups II and III in those animals prepared for nerve harvest at the day of 7,1...
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