Detection Of AgNORs Proteins In Peripheral Blood Lymphocytes In Children With Malignancies

Background T Lymphocytes play an important role in immunoloregulation and immunoloreaction. Argyrophilic nucleolar organizer regions associated proteins (AgNORs proteins) of lymphocytes are a set of acid non-histone, which re-fleet the level of transcription activities of the rRNA genes as an important marker in rDNA transcription activities . Malignant tumor cells release inhibitive factor damaging immune system so that expression activities of acid non-histone regulating rRNA synthesis are specificly inhibited and the amount of argyrophilic nucleolar organizer regions associated proteins becomes low.Purpose To analyze the AgNORs expression in the peripheral blood T Lymphocytes in children with malignancies , and its changes in different status.Method Peripheral blood T Lymphocytes from normal children(n=107), children with benign diseases (n=29), and children with malignancies(n=71) were cultivated , stimulated and stained by a specific silver staining technique. The mean proportion betweenAgNORs areas and nucleus area (1.S%) was analyzed with a KL-2 computer-assisted image analysis system for microscopic image analysis provided by Beijing Janerkang armamentarium company limited. Statistical analysis was performed.Results (l)The average I.S% values were (7.34 ?0.94)%, (7.53 ?1.73)% and (6.49 ?1.50)%, respectively for normal children, children with benign diseases and children with malignancies, respectively. Statistical analysis revealed there was significant difference between children with malignancies and normal children (p=0.0001<0.05). While no significant difference was observed between normal children and children with benign diseases(p=0.76>0.05). (2) The average I.S% value in children with cancer CR phase was higher than that in those without CR(6.92?.54 vs 6.04?.25, t=2.65, p=0.01<0.05). (3)AgNORs in children postchemotherapy was lower than that in those prechemotherapy (5.28?.86 vs 6.38?.52,t=2.67, p=0.01<0.05). (4) Significant diffierence was observed between normal children and cancer patients without infection (p<0.05),and so dose for cancer patients with infection, compared with those without infection(p<0.05). (5)There was no significant difference among patients with differentcancers(F=1.41,p>0.05).Conclusion T cell AgNORs value in children with malignancies was lower than that in normal children; There was no significant difference between normal children and children with benign diseases ; The tumor burden and chemotherapy might be important factors influencing AgNORs value ; Infection might trigger immunological response so that higher AgNORs value observed; AgNORs detection was a potential marker for indication of cellular immunological function .