Optimization Of LDL-Adsorbent And Treatment Of Rabbit With Hyperlipidemia By LDL Apheresis

The association between hyperlipidemia and atherosclerosis is unequivocal. And low density lipoprotein (LDL) subclasses differ concerning their atherogenic potential. Small, dense LDL are more atherogenic than large, buoyant LDL. LDL apheresis is a better treatment option for patients with refractory hyperlipidemia (especially familial hypercholesterolemia) who fall to respond adequately to diet and drug therapy In order to develop a better adsorbent, we have designed several different type of adsorbent (dextran sulfate adsorbent, dextran adsorbent with amphiphilic iigands and cellulose adsorbent with amphiphilic ligands). All of the adsorbent were compared concerning their efficacy by mixed plasma in vitro. The result shows cellulose adsorbent with amphiphilic ligands is preferable to others (P <0 05). And LDL, TC, TG were selectively removed (P <0.01) without the substantial reduction of high density lipoprotein.The effects of cellulose adsorbent with amphiphilic ligands on plasma lipid of rabbit with hyperlipidemia were observed. The sorbent removed TC, TG and LDL (P <0.01), leaving HDL intact. And on the basis of 3 LDL subtractions (d= 1.020-1.060g/ml) isolated by density gradient ultracentrifugation the LDL-density profile was determined in each rabbit with hyperlipidemia before and after apheresis. All of the LDL-subclasses were reduced. But there was a relative increase of LDL-subfraction 1 and 2 and a concomitant decrease of LDL-subfraction 3 after perfusion. The extent of small, dense LDL reduction correlated with the preapheresis concentration of LDL but not with that of tirglyceride. The physical or chemical character of sLDL maybe contribute to the result that the degree of small, dense LDL reduction was more prominent than that of large, buoyant LDL. In conclusion, the novel adsorbent not only decreases TC, TG and LDL mass, but also improves LDL profile.