| Objective To observe osteogenicity of composite grafting in vivo of autologous marrow mesenchymal stem cells (MS C) of rabbits and search for ideal matrix of MSC. Methods The autologous MSCs of rabbits were isolated from bone marrow, grown in culture, and loaded onto demineralized bovine cancellous bone (DBCB) and calcium phosphate cement (CPC) respectively and then implanted separately into the left sacrospinal muscle of two groups of rabbits (Group A with MSC+DBCB and Group B with MSC+CPC).At the same time, the pure DBCB and CPC were separately implanted into the right sacrospinal muscle of two groups of rabbits (Group A with pure DBCB and Group B with pure CPC) as the blank control. Sixteen weeks later, the animals were killed and the 2 specimens were harvested and observed for the formation of bone. the histological sections of specimens were for this purpose stained and observed. Results In Group A (of five rabbits),abundant new bone was found to have formed in all specimens of MSC-DBCB while the implanted pure DBCB was wholly or mostly absorbed degraded.In Group B (of five rabbits),no new bone was found to have formed in any specimens of MSC-CPC or pure CPC and none of the implanted spelimens was markedly absorbed. Conclusion The autologous MSC loaded onto appropriate matrix can form bone in vivo and DBCB is the satisfactory matrix for MCS. |
