Study On The Distribution Of Irp1,irp3 And Irp4 Genes Located On Yersinia Enterocolitia "High-Pathogenicity Island" Among Escherichia Coli Strains

In this study, we investigate the distributions of irpl, irp3 and irp4 genes located Yersinia enterocolitia HPI by PCR amplification and situ dot hybridization assay. This is the first study on the distributions of irpl, irp3, and irp4 genes of the HPI located on Yersinia enterocolitia among so many E.coli strains.And we describe and analysis the results to provide a necessary experimental base on horizontal transfer of the HPI between Y enterocolitia and some E.coli strains. With goldkey software, we design three pairs primers of irpl, irp3 and ipr4 genes of the Yen HPI from Genebank. And detect the distributions of the three genes among 106 E.coli strains by PCR smplification and situ dot hybridization method respectively. Both the two results detected in this study are identical(the results of PCR): (1) Thirteen strains are positive for irpl gene(12.3%); (2)Ninty-two strains are positive for irp3 gene(86.8%); (3)Sixty-five strains are positive for irp4 gene(6 1.3%). These results demonstrate that the three genes exist among E. ccli strains. Accordingly, the author conclude that:Firstly ,the horizontal tranfers of irpl, irp3, and irp4 genes between Yen and some E.coli strains do exist. Secon dly, the deletion rate of irpl gene is far higher than that of irp3 gene or irp4 gene. Thirdly, both PCR and hybridization technologies are feasible, sensitive and effective to detect genes.