The Expression Of Fas Antigen And Fas Ligand On Early Stage Of Hepatic Damage In Diabetic Tree Shrews

The expression of Fas antigen and Fas ligand on earIy stage ofhepatic damage in diabetic tree shrewsAbstractObjcctive To stUdy the expression of Fas antigen and Fas ligand on earlystage of hepatic damage in diabetic tree shrews and its significance. Methods39 tree shIews were diVided randomly into normal control group (n=12) andtreaAnent group (n=27). Tree shrew5 of treatInent group were injected of vein(i.v.) with streptozocin (Sro to induce diabetic damage. Sis weeks later, thetree shrews maintained fasting blood glucose (FBG) at the level of 311.1rIunoW were divided into diabetes grouP(n=10), and the onesmaintained FBG <11.1mmol/L into STZ control grouP(n=8). Theconcentration of serum ALT TBIL and Fas were tested before and after STZinjection 2, 4 and 6 weeks. All animals were performed hepatectomy after i.v.STZ fOr 6 weeks. All of the liver biopsies were observed with light.microscoPe and e1ectron microsCOpe. The anti-Fas and anti-Fas ligand wasstUdied irnmunohistochemically. ResuIt The coocentration of serum ALT indiabetes group increased gradually. But there was no significant difference inthe concentrations of serum ALT and TBlL among the three groups befOreand after i.v. STZ. The concentration of serum Fas in diabetes groupincreased gradually, with the significant dtherence among the levels of beforetreatment and other tWo groups at the time points of 4 and 6 week (P<0.05).The fOllowing changes were observed with light microscope: l. ballooningdegeneratioll, 2. cloudy swelling, 3. eosinophilic body, 4. spotty necrosis, 5.2vacuolar degeneration in cytoplasm, 6. inflammatory infiltration in portal area, 7. increased glycogen granules in cytoplasm. And the following changes were observed under electron microscope: 1. glycogen granules in cytoplasm and nudes increasing, 2. rough endoplasmic reticulum degranulation, 3. smooth endoplasmic reticulum expansion, 4. configuration of mitochondrial illegibility, 5. fatty degeneration. The expression degrees of both FasAg and FasL in liver tissues of diabetes group were stronger than those of the other two groups (P