The Regulation Of NTF On The Developing Nerve In The Maxillofacial Region And The Effects Of RhBMP-2 On The Generation Of Peripheral Nerve

Bone morphogenetic proteins(BMPs) family is a group of secreted polypeptides that regulate a diverse array of developmemtal and biological pocesses. These factors signal through heteromeric complexes of transmembrane type I and type II Ser/Thr kinase receptors. Within this complex the type 11 receptor kinase activates the type I receptor kinase, which subsequently propagates signals to the Smads. GDNF(Glial cell-derived neurotrophic factor), originally founded by Lin et al in glial cell line B49, a neurotrophic factor that promotes the survival of many different kinds of neurons and influence axonal and dendritic growth and synaptic function. These diverse effects are mediated via multicomponent receptors consisting of the Ret receptor tyrosine kinase plus one of two structurally related GPI-linked receptors, GFRct-l and GFRc-2. To further understanding the roles of BMP and GDNF during the developmental and repairing processes of nerves, we conducted the following research work: At ElO, 14, 18 and 2 day after born, we examined the express of BMP, GDNF and GFR in maxillofacial region nerves with immunohistochemistry. BMP was detected at every time point, whereas GDNF and (IFR was weakly stained in late stage of development. The results suggested BMP may play an important role throughout the development and GDNF/GFR may act after the establishment of nerve-target organ connection. The effects of BMP-2 on Schwann's cell in vitro was studied and the results suggested rhBMP-2 could enhance the proliferation of Schwann's cell and could upregulate the secretion of neu2rotrophic factor. The results of BMP-2 on peripheral nerve regeneration showed BMP-2 could sufficiently stimulate neurite outgrowth from ganglia and Schwann's cell proliferation and migration.