Experimental Study Of Mechanism Of Brain Edema After Intracerebral Hemorrhage And Treatment Effect Of Hirudo

Intracerebral hemorrhage is one of diseases which are common and severe in department of neurology. Intracerebral hemorrhagee constitutes about 10% of stroke patients. There are high declining fatality and disability in patients of intracerebral hemorrhage. Brain edema after intracerebral hemorrhage, a secondary reaction, may deteriorate neurological function. In the present study, brain edema induced by some factors of blood clot formation and blood dissolveing is paid attention. In this study, we sought to determin the role of thrombin in brain edema formation after experimental intracerebral hemorrhage. We also examin the role of hirudo in treatment of intracerebral hemorrhage.Material and MethodsOne hundred and fourteen adult male Sprague Dawley rats, each weighing between 300 and 350g, were used in this study. They were anesthetized with an intraperitoneal injection of 10% chlorali hydras ( 300mg/100g). A rat was positioned in a stereotactic frame, and a needle was inserted into the right caudate nucleus of the rat. Whole blood, heparinized blood, thrombin and saline were infused into the right caudate nucleus with a microinfusion pump at a rate of 15-20ul/min. Twenty rats were treated for 5 days with*hirudo before and after making model(250mg/kg,ql2h,po). The mark of defective neurologic function (MDNF) was measured on 24h,4Experimental study of mechanism of brain edema after intracerebral hemorrhage and treatment effect of hirudo (ABSTRACT)He YupingHuang JianzhenBackground and ObjectiveIntracerebral hemorrhage is one of diseases which are common and severe in department of neurology. Intracerebral hemorrhagee constitutes about 10% of stroke patients. There are high declining fatality and disability in patients of intracerebral hemorrhage. Brain edema after intracerebral hemorrhage, a secondary reaction, may deteriorate neurological function. In the present study, brain edema induced by some factors of blood clot formation and blood dissolveing is paid attention. In this study, we sought to determin the role of thrombin in brain edema formation after experimental intracerebral hemorrhage. We also examin the role of hirudo in treatment of intracerebral hemorrhage.Material and MethodsOne hundred and fourteen adult male Sprague Dawley rats, each weighing between 300 and 350g, were used in this study. They were anesthetized with an intraperitoneal injection of 10% chlorali hydras ( 300mg/100g). A rat was positioned in a stereotactic frame, and a needle was inserted into the right caudate nucleus of the rat. Whole blood, heparinized blood, thrombin and saline were infused into the right caudate nucleus with a microinfusion pump at a rate of 15-20ul/min. Twenty rats were treated for 5 days with*hirudo before and after making model(250mg/kg,ql2h,po). The mark of defective neurologic function (MDNF) was measured on 24h,4cerebral edema when heparinized blood or saline is infused into right caudate nucleus in rats. It reveals that thrombin may be an important factor in brain edema formation after experimental intracerebral hemorrhage. Hirudo may attenuate brain edema after intracerebral hemorrhage and thrombin. Hirudo may treat experimental intracerebral hemorrhage of rats.