| Objective: The pathogeny and pathogenesis of type 2 diabetes mel I itus are unknown, and the relationship between type 2 diabetes or vascular complication of diabetes and angiotensin I converting enzyme (ACE) gene insertion /deletion (I/O) polymorphism are controversial. To evaluate relationship between ACE gene and type 2 diabetes and vascular complications, we study ACE gene I/O polymorphism susceptibility to type 2 diabetes. Methods: Polymerase Chain Reaction (PCR) was used to determine the I/O polymorphism genotypes in intron 16 of the ACE gene in 120 cases of type 2 diabetes patients and 100 healthy controls. Results: The frequencies of DD genotype and 0 allele of ACE gene were much higher in the type 2 diabetes patients and diabetes nephropathy (ON) than in controls, but TI genotype and I allele抯 frequencies were much lower in these patients. Compared to thos without CHD, the frequency of ID genotype in type 2 diabetes patients with CHD was much higher, butllgenotype frequency was much lower. The distribution of DD~ ID. II genotypes and D~ I alleles of the ACE gene had no any difference among type 2 diabetes patients with or without diabetic retinopathy (DR) and controls, the same as the compare among type 2 diabetes patients with or without HP and controls. Conclusion: 1. The I/O polymorphism of the ACE gene was associated with type 2 diabetes~ DN and diabetes with CHD in Guangxi region. 2. DO genotype and D allele of ACE gene were associated with the susceptibility to type 2 diabetes and DN. DD genotype and D allele were risk factors of type 2 diabetes mel I itus patients 3 and those with DN. H genotype and I at IeIe were markers of reduce risk in type 2 diabetes and thee with DN or CHD. ID g notype and D allele w re risk factors of type 2diabeteswith CHD. 3. The I/Dpolymorphismsof theACEgenewere associated with type 2 diabetes with DR or HP in Guangxi region. |
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