Study Of A Data Acquisition And Analysis System For Optical Spr Biosensor Using LabVIEW Virtual Instrument

Optical Surface Plasmon Resonance (SPR) is a new biophysical analytical technology which has been used in a wide range of fields including medicine, clinic diagnosis, environmental monitor and food detection etc. Optical SPR biosensor has the advantage of label-free detection, real-time monitoring, and non-invasive analysis. However, regularly, the data acquisition and analysis system used for optical SPR bio-analyzer, is made using a special imbedded data acquisition card in order to acquire and process mass opto-electronic data, and its software based PC is only used for transferring and displaying data, and its communication interface with PC is RS-232 standard interface, which is not beneficial for miniaturization and not fulfillable for instrumentation on PC. With the development of virtual instrument, it is more effective for data management and easier for instrument maintenance that using virtual instrument technology to realize data transmission and acquisition, data format conversion, resonance photo-position solution, parameter setting and other data processes. Therefore, it has important and practical value to study a data acquisition and analysis system for optical SPR bio-analyzer using virtual instrument technology.Introduction of virtual instrument conception to the innovative design of optical SPR bio-analyzer is the key point in this system. For the design of a data acquisition and analysis system for optical SPR biosensor, this paper systematically makes a study of operation principle and implementation method of optical SPR biosensor, state of the art and developing direction of optical SPR bio-analyzer, the role of virtual instrument technology in measurement instrument and virtual instrument implementation technique; and presents the design and development method of a data acquisition and analysis system for optical SPR biosensor, which uses a TSPR1K23 integrated biosensor made from Texas Instruments to obtain bio-signal and a embedded microprocessor PIC24HJ32GP302 to acquire opto-electronic photooptical signal; communication interface is designed with CP2102 a USB-UART bridging chip, and virtual instrument panel-HPSPR8000 is programmed by LabVIEW which accomplishes front data sample and data process and realizes functions such as data acquisition, data process, real-time monitoring, data storage, etc. In the paper, experiments of ethanol with different volume fraction are done to verify the software-HPSPR8000, the results of which indicate that the virtual instrument panel-HPSPR8000 is easy for operation and able to friendly monitor SPR resonance curves and response curves, and detection sensitivity reaches up to 1×10^-6 RIU.