Study On Gastrointestinal Parasites And Escherichia Coli In Non-human Primates Of Some Zoological Gardens In China

As valuable natural resources, the wild animals should be protected by all mankind as the common responsibility. With the most common gastrointestinal parasites' and the most serious pathogens E.coli presence, many species of non-human primates belong to the world's endangered species. The present work was performed to survey the gastrointestinal parasites of non-human primate from 14 zoogical gardens in China, and also to finish the isolation, identification and susceptibility test of intestinal E. coli in those animals from 9 zoogical gardens.Sequential precipitation method, saturated magnesium sulfate flotation method, and saturated sugar solution flotation method were used to investigate the infection of intestinal parasites.951 non-human primates, including 8 family,25 genus,48 species from 14 Zoos around the country, were carried out the inspection of gastrointestinal parasites. As a result, nine kinds of gastrointestinal parasites were detected. And the prevalence of parasitism as follows, Strongyloides fulleborni (2.21%), Trichuris trichiura(14.20%), Oesophagostomum sp. (3.58%), Enterobius sp. (0.84%), Ascaris sp. (0.32%), Hymenolepis nana (0.95%), Eimeria sp. (0.42%), Entamoeba spp. (0.11%) and Giardia duodenalis (0.32%). There was no significant difference in Trichuris trichiura prevalence between different zoos (p=0.509), but there was significant difference between different animals species (p<0.001). There was significant difference in Trichuris trichiura prevalence between Rhinopithecus roxellanae, which has the highest infection rate to Trichuris trichiura,and other 14 species positive primates (p<0.001),however,there was no significant difference among the other positive primates(p=0.471).A total of 330 fecal samples were collected and examined for intestinal E. coli. 169 E. coli were isolated and identified by a series of tests, including isolation of bacteria, biochemical tests, etc.According to the U.S.Committee for Clinical Laboratory Standards (CLSI), antimicrobial susceptibility test, against 22 kinds of common antibiotics and other antibiotics, were carried out to the 169 E.coli which isolated. Our results indicated that the 169 E.coli showed different degrees of drug resistance. They were of the highest resistance to carbenicillin(drug resistance rate:90.53%),followed by sulfamethoxazole(82.25%).They had the highest sensitivity to Imipenem (sensitivity rate:93.49%), followed by netilmicin (81.66%); While moderately sensitive to cefotaxime(sensitivity rate:56.80%), followed by azithromycin (40.24%).The phenomenon of multiple drug resistance occurred in E.coli.114 of them were indentified to resistant three to nine kinds of antibiotics; 51 of them resistant more than ten kinds of antibiotics. There was significant difference in drug resistance rate between TY zoo and the other 8 zoos (p<0.001);while there was no significant difference between the others.